3S8R
Crystal Structures of Glutaryl 7-Aminocephalosporanic Acid Acylase: Insight into Autoproteolytic Activation
Replaces: 1OQZExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.06290 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 225.386, 68.806, 112.756 |
| Unit cell angles | 90.00, 97.51, 90.00 |
Refinement procedure
| Resolution | 26.410 - 2.500 |
| R-factor | 0.1558 |
| Rwork | 0.153 |
| R-free | 0.19990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.217 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.830 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 59274 | |
| Completeness [%] | 94.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.5 M ammonium sulfate, 0.1 M Tris-HCl (pH 8.5), and 12% glycerol , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
