3ROV

Insulin's biosynthesis and activity have opposing structural requirements: a new factor in neonatal diabetes mellitus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I...	Insulin	polymer	21	2383.7	6	UniProt (P01308) In PDB	Homo sapiens	Insulin A chain
2	B, D, F, H, J...	Insulin	polymer	30	3462.0	6	UniProt (P01308) Pfam (PF00049) In PDB	Homo sapiens	Insulin B chain
3	A, C, E, G, I...	PHENOL	non-polymer		94.1	6	Chemie (IPH)
4	B, D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5	B, H	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
6		water	water		18.0	186	Chemie (HOH)

Sequence modifications

B, D, F, H, J, L: 1 - 30 (UniProt: P01308)

PDB	External Database	Details
Dal 20	Gly 44	engineered mutation
Dal 23	Gly 47	engineered mutation
Lys 28	Pro 52	engineered mutation
Pro 29	Lys 53	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	35074.2
Non-Polymers*	Number of molecules	10
	Total formula weight	766.4
All*	Total formula weight	35840.6

*Water molecules are not included.