3ROV
Insulin's biosynthesis and activity have opposing structural requirements: a new factor in neonatal diabetes mellitus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-10-09 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.662, 61.772, 46.038 |
Unit cell angles | 90.00, 105.50, 90.00 |
Refinement procedure
Resolution | 44.360 - 2.300 |
Rwork | 0.218 |
R-free | 0.30700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1znj |
RMSD bond angle | 0.008 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.360 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.057 | 0.232 |
Number of reflections | 10607 | |
<I/σ(I)> | 12.1 | 32.8 |
Completeness [%] | 95.9 | 91.1 |
Redundancy | 2.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 298 | 0.05 M sodium citrate, 1% phenol, 0.04% zinc acetate, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |