3RJ1

Architecture of the Mediator Head module

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, H, O (A, H, O)	Mediator of RNA polymerase II transcription subunit 11	polymer	131	15240.8	3	UniProt (Q99278) Pfam (PF10280)	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	Mediator complex subunit 11
2	B, I, P (B, I, P)	Mediator of RNA polymerase II transcription subunit 17	polymer	583	66912.1	3	UniProt (P32569) Pfam (PF10156)	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	Mediator complex subunit 17, Suppressor of RNA polymerase B 4
3	C, J, Q (C, J, Q)	Mediator of RNA polymerase II transcription subunit 8	polymer	223	25484.8	3	UniProt (P38304) Pfam (PF10232)	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	Mediator complex subunit 8
4	D, K, R (D, K, R)	Mediator of RNA polymerase II transcription subunit 22	polymer	121	14110.2	3	UniProt (P32570) Pfam (PF06179)	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	Mediator complex subunit 22, Suppressor of RNA polymerase B 6
5	E, L, S (E, L, S)	Mediator of RNA polymerase II transcription subunit 18	polymer	275	31128.7	3	UniProt (P32585) Pfam (PF09637)	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	Mediator complex subunit 18, Suppressor of RNA polymerase B 5
6	F, M, T (F, M, T)	Mediator of RNA polymerase II transcription subunit 20	polymer	210	23105.7	3	UniProt (P34162) Pfam (PF08612)	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	Hyper-recombination suppressor protein 2, Mediator complex subunit 20, Suppressor of RNA polymerase B 2
7	G, N, U (G, N, U)	Mediator of RNA polymerase II transcription subunit 6	polymer	295	33501.3	3	UniProt (P38782) Pfam (PF04934)	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	Mediator complex subunit 6
8	AA, AB, AC, AD, BA... (B, H, L, R, M...)	SELENIUM ATOM	non-polymer		79.0	98	Chemie (SE)

Sequence modifications

A, H, O: 1 - 131 (UniProt: Q99278)

PDB	External Database	Details
Gly 16	Thr 16	engineered mutation
Ser 17	Met 17	engineered mutation

B, I, P: 109 - 616 (UniProt: P32569)

PDB	External Database	Details
Mse 98	-	expression tag
His 99	-	expression tag
His 100	-	expression tag
His 101	-	expression tag
His 102	-	expression tag
His 103	-	expression tag
His 104	-	expression tag
His 105	-	expression tag
His 106	-	expression tag
His 107	-	expression tag
His 108	-	expression tag

B, I, P: 669 - 687 (UniProt: P32569)
E, L, S: 1 - 108 (UniProt: P32585)
E, L, S: 141 - 307 (UniProt: P32585)

Contents of the asymmetric unit

Polymers	Number of chains	21
	Total formula weight	628450.6
Non-Polymers*	Number of molecules	98
	Total formula weight	7738.1
All*	Total formula weight	636188.7

*Water molecules are not included.