3RJ1
Architecture of the Mediator Head module
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97948 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 265.914, 265.914, 319.948 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.911 - 4.300 |
R-factor | 0.3477 |
Rwork | 0.345 |
R-free | 0.37260 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.544 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 4.300 |
Rmerge | 0.084 |
Number of reflections | 89531 |
<I/σ(I)> | 8.9 |
Completeness [%] | 92.6 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 0.1 M Tris-HCl (pH 7.6) containing 10-12.5% (w/v) PEG-6K, and 0.4 M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |