3RHJ
Crystal structure of the E673A mutant of the C-terminal domain of RAT 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE in complex with co-purified NADP
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Aldehyde dehydrogenase 1 family, member L1 | polymer | 517 | 56563.5 | 4 | UniProt (Q5HZB2) Pfam (PF00171) UniProt (by SIFTS) (P28037) In PDB | Rattus norvegicus (brown rat rat rats) | |
2 | D, A, B, C | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 743.4 | 4 | Chemie (NAP) | |||
3 | C, D, A, B | SULFATE ION | non-polymer | 96.1 | 24 | Chemie (SO4) | |||
4 | C, D, A, B | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
5 | water | water | 18.0 | 1455 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 397 - 902 (UniProt: Q5HZB2)
PDB | External Database | Details |
---|---|---|
Met 386 | - | expression tag |
Arg 387 | - | expression tag |
Gly 388 | - | expression tag |
Ser 389 | - | expression tag |
His 390 | - | expression tag |
His 391 | - | expression tag |
His 392 | - | expression tag |
His 393 | - | expression tag |
His 394 | - | expression tag |
Thr 395 | - | expression tag |
Thr 396 | - | expression tag |
Ala 673 | Glu 673 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 226254.0 | |
Non-Polymers* | Number of molecules | 32 |
Total formula weight | 5647.5 | |
All* | Total formula weight | 231901.5 |