3RHJ

Crystal structure of the E673A mutant of the C-terminal domain of RAT 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE in complex with co-purified NADP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Aldehyde dehydrogenase 1 family, member L1	polymer	517	56563.5	4	UniProt (Q5HZB2) Pfam (PF00171) UniProt (by SIFTS) (P28037) In PDB	Rattus norvegicus (brown rat rat rats)
2	D, A, B, C	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	4	Chemie (NAP)
3	C, D, A, B	SULFATE ION	non-polymer		96.1	24	Chemie (SO4)
4	C, D, A, B	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
5		water	water		18.0	1455	Chemie (HOH)

Sequence modifications

A, B, C, D: 397 - 902 (UniProt: Q5HZB2)

PDB	External Database	Details
Met 386	-	expression tag
Arg 387	-	expression tag
Gly 388	-	expression tag
Ser 389	-	expression tag
His 390	-	expression tag
His 391	-	expression tag
His 392	-	expression tag
His 393	-	expression tag
His 394	-	expression tag
Thr 395	-	expression tag
Thr 396	-	expression tag
Ala 673	Glu 673	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	226254.0
Non-Polymers*	Number of molecules	32
	Total formula weight	5647.5
All*	Total formula weight	231901.5

*Water molecules are not included.