3RHJ
Crystal structure of the E673A mutant of the C-terminal domain of RAT 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE in complex with co-purified NADP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Aldehyde dehydrogenase 1 family, member L1 | polymer | 517 | 56563.5 | 4 | UniProt (Q5HZB2) Pfam (PF00171) UniProt (by SIFTS) (P28037) | Rattus norvegicus (brown rat rat rats) | |
| 2 | DA, E, M, U (D, A, B, C) | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 743.4 | 4 | Chemie (NAP) PubChem (5885) PubChem (46936685) PubChem (71717786) PubChem (4412) PubChem (90473931) PubChem (91972139) PubChem (145946263) PubChem (153311489) PubChem (174001641) PubChem (165382010) | |||
| 3 | AA, BA, EA, F, FA... (C, D, A, B) | SULFATE ION | non-polymer | 96.1 | 24 | Chemie (SO4) | |||
| 4 | CA, JA, L, T (C, D, A, B) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) PubChem (753) | |||
| 5 | KA, LA, MA, NA (A, B, C, D) | water | water | 18.0 | 1455 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 397 - 902 (UniProt: Q5HZB2)
| PDB | External Database | Details |
|---|---|---|
| Met 386 | - | expression tag |
| Arg 387 | - | expression tag |
| Gly 388 | - | expression tag |
| Ser 389 | - | expression tag |
| His 390 | - | expression tag |
| His 391 | - | expression tag |
| His 392 | - | expression tag |
| His 393 | - | expression tag |
| His 394 | - | expression tag |
| Thr 395 | - | expression tag |
| Thr 396 | - | expression tag |
| Ala 673 | Glu 673 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 226254.0 | |
| Non-Polymers* | Number of molecules | 32 |
| Total formula weight | 5647.5 | |
| All* | Total formula weight | 231901.5 |
