3RHJ
Crystal structure of the E673A mutant of the C-terminal domain of RAT 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE in complex with co-purified NADP
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0016620 | molecular_function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 36 |
| Details | BINDING SITE FOR RESIDUE NAP A 101 |
| Chain | Residue |
| A | VAL570 |
| A | GLY634 |
| A | GLN635 |
| A | PHE648 |
| A | THR649 |
| A | GLY650 |
| A | SER651 |
| A | VAL654 |
| A | HIS657 |
| A | ILE658 |
| A | ALA673 |
| A | ILE571 |
| A | LEU674 |
| A | GLY675 |
| A | CYS707 |
| A | GLU804 |
| A | PHE806 |
| A | LEU834 |
| A | PHE872 |
| A | HOH1095 |
| A | HOH1110 |
| A | HOH1111 |
| A | TRP573 |
| A | HOH1166 |
| A | HOH1257 |
| A | HOH1262 |
| A | HOH1265 |
| A | HOH1276 |
| A | HOH1295 |
| A | HOH1332 |
| A | ASN574 |
| A | LYS597 |
| A | ALA599 |
| A | GLN600 |
| A | SER629 |
| A | GLY630 |
| site_id | AC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 A 102 |
| Chain | Residue |
| A | LYS852 |
| C | ARG554 |
| site_id | AC3 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE SO4 A 103 |
| Chain | Residue |
| A | ARG636 |
| site_id | AC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A 104 |
| Chain | Residue |
| A | ARG551 |
| A | ARG554 |
| C | LYS852 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A 105 |
| Chain | Residue |
| A | ASN548 |
| A | HOH1138 |
| B | GLN528 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 106 |
| Chain | Residue |
| A | ASN738 |
| A | GLU741 |
| A | ARG781 |
| C | HOH1285 |
| site_id | AC7 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE SO4 A 107 |
| Chain | Residue |
| A | LYS669 |
| A | LEU893 |
| A | ARG894 |
| A | ILE895 |
| A | HOH1144 |
| A | HOH1288 |
| B | GLY881 |
| B | LYS882 |
| B | HOH1001 |
| site_id | AC8 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL A 108 |
| Chain | Residue |
| A | TYR575 |
| A | MET578 |
| A | TRP582 |
| A | ASN706 |
| A | CYS707 |
| A | THR866 |
| A | HOH1348 |
| site_id | AC9 |
| Number of Residues | 36 |
| Details | BINDING SITE FOR RESIDUE NAP B 101 |
| Chain | Residue |
| B | HOH1314 |
| B | HOH1320 |
| B | VAL570 |
| B | ILE571 |
| B | TRP573 |
| B | ASN574 |
| B | LYS597 |
| B | ALA599 |
| B | GLN600 |
| B | SER629 |
| B | GLY630 |
| B | GLY634 |
| B | GLN635 |
| B | PHE648 |
| B | THR649 |
| B | GLY650 |
| B | SER651 |
| B | VAL654 |
| B | HIS657 |
| B | ILE658 |
| B | ALA673 |
| B | LEU674 |
| B | GLY675 |
| B | CYS707 |
| B | GLU804 |
| B | PHE806 |
| B | LEU834 |
| B | PHE872 |
| B | HOH1040 |
| B | HOH1060 |
| B | HOH1143 |
| B | HOH1149 |
| B | HOH1176 |
| B | HOH1264 |
| B | HOH1281 |
| B | HOH1297 |
| site_id | BC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 B 102 |
| Chain | Residue |
| B | ARG554 |
| D | LYS852 |
| D | HOH1179 |
| site_id | BC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 B 103 |
| Chain | Residue |
| B | ARG636 |
| B | PRO641 |
| B | HOH1270 |
| B | HOH1319 |
| site_id | BC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 B 104 |
| Chain | Residue |
| B | ARG551 |
| B | ARG554 |
| D | TYR848 |
| site_id | BC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 B 105 |
| Chain | Residue |
| A | GLN528 |
| B | ASN548 |
| B | HOH1095 |
| B | HOH1137 |
| B | HOH1251 |
| B | HOH1253 |
| site_id | BC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 B 106 |
| Chain | Residue |
| B | THR445 |
| B | ASN738 |
| B | GLU741 |
| B | ARG781 |
| B | HOH1141 |
| B | HOH1279 |
| site_id | BC6 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE SO4 B 107 |
| Chain | Residue |
| A | GLY881 |
| A | LYS882 |
| A | HOH1002 |
| B | LYS669 |
| B | LEU893 |
| B | ARG894 |
| B | ILE895 |
| B | HOH1292 |
| B | HOH1339 |
| site_id | BC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL B 108 |
| Chain | Residue |
| B | TYR575 |
| B | ASN706 |
| B | ASN864 |
| B | THR866 |
| B | HOH1344 |
| site_id | BC8 |
| Number of Residues | 36 |
| Details | BINDING SITE FOR RESIDUE NAP C 101 |
| Chain | Residue |
| C | VAL570 |
| C | ILE571 |
| C | PRO572 |
| C | TRP573 |
| C | ASN574 |
| C | LYS597 |
| C | ALA599 |
| C | GLN600 |
| C | GLY630 |
| C | GLY634 |
| C | GLN635 |
| C | PHE648 |
| C | THR649 |
| C | GLY650 |
| C | SER651 |
| C | VAL654 |
| C | HIS657 |
| C | ILE658 |
| C | ALA673 |
| C | LEU674 |
| C | GLY675 |
| C | CYS707 |
| C | GLU804 |
| C | PHE806 |
| C | LEU834 |
| C | PHE872 |
| C | HOH1056 |
| C | HOH1065 |
| C | HOH1087 |
| C | HOH1091 |
| C | HOH1132 |
| C | HOH1133 |
| C | HOH1218 |
| C | HOH1312 |
| C | HOH1334 |
| C | HOH1381 |
| site_id | BC9 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 C 102 |
| Chain | Residue |
| A | ARG554 |
| C | LYS852 |
| site_id | CC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 C 103 |
| Chain | Residue |
| C | ARG636 |
| C | PRO641 |
| site_id | CC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 C 104 |
| Chain | Residue |
| C | ARG551 |
| C | ARG554 |
| site_id | CC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 C 105 |
| Chain | Residue |
| C | ASN548 |
| C | HOH1233 |
| C | HOH1243 |
| C | HOH1283 |
| C | HOH1390 |
| D | GLN528 |
| site_id | CC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 C 106 |
| Chain | Residue |
| A | LYS415 |
| C | ASN738 |
| C | ARG781 |
| C | HOH1039 |
| C | HOH1328 |
| site_id | CC5 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE SO4 C 107 |
| Chain | Residue |
| C | LYS669 |
| C | LEU893 |
| C | ARG894 |
| C | ILE895 |
| C | HOH1072 |
| C | HOH1140 |
| C | HOH1255 |
| D | GLY881 |
| D | LYS882 |
| D | HOH1002 |
| site_id | CC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 C 108 |
| Chain | Residue |
| A | LEU416 |
| A | THR417 |
| C | LYS415 |
| C | ARG742 |
| site_id | CC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL C 109 |
| Chain | Residue |
| C | TYR575 |
| C | TRP582 |
| C | ASN706 |
| C | CYS707 |
| C | THR866 |
| C | HOH1330 |
| site_id | CC8 |
| Number of Residues | 35 |
| Details | BINDING SITE FOR RESIDUE NAP D 101 |
| Chain | Residue |
| D | VAL570 |
| D | ILE571 |
| D | TRP573 |
| D | ASN574 |
| D | LYS597 |
| D | ALA599 |
| D | GLN600 |
| D | SER629 |
| D | GLY630 |
| D | GLY634 |
| D | GLN635 |
| D | PHE648 |
| D | THR649 |
| D | GLY650 |
| D | SER651 |
| D | VAL654 |
| D | HIS657 |
| D | ILE658 |
| D | ALA673 |
| D | LEU674 |
| D | GLY675 |
| D | CYS707 |
| D | GLU804 |
| D | PHE806 |
| D | LEU834 |
| D | PHE872 |
| D | HOH1035 |
| D | HOH1049 |
| D | HOH1085 |
| D | HOH1135 |
| D | HOH1198 |
| D | HOH1284 |
| D | HOH1298 |
| D | HOH1307 |
| D | HOH1319 |
| site_id | CC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 D 102 |
| Chain | Residue |
| B | ASP851 |
| B | LYS852 |
| B | HOH1125 |
| D | ARG554 |
| site_id | DC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 D 103 |
| Chain | Residue |
| D | ARG636 |
| D | PRO641 |
| D | HOH1338 |
| site_id | DC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 D 104 |
| Chain | Residue |
| D | ARG551 |
| D | ARG554 |
| site_id | DC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 D 105 |
| Chain | Residue |
| D | ASN738 |
| D | GLU741 |
| D | ARG781 |
| D | HOH1153 |
| site_id | DC4 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE SO4 D 106 |
| Chain | Residue |
| C | GLY881 |
| C | LYS882 |
| C | HOH1006 |
| D | LYS669 |
| D | LEU893 |
| D | ARG894 |
| D | ILE895 |
| D | HOH1227 |
| D | HOH1309 |
| site_id | DC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL D 107 |
| Chain | Residue |
| D | MET578 |
| D | ASN706 |
| D | THR866 |
| D | HOH1351 |
Functional Information from PROSITE/UniProt
| site_id | PS00070 |
| Number of Residues | 12 |
| Details | ALDEHYDE_DEHYDR_CYS Aldehyde dehydrogenases cysteine active site. FfNKGENCIAAG |
| Chain | Residue | Details |
| A | PHE700-GLY711 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1940 |
| Details | Region: {"description":"Aldehyde dehydrogenase domain","evidences":[{"source":"PubMed","id":"10585460","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"PubMed","id":"17302434","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"17302434","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"21540484","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"2O2Q","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3RHO","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 44 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"17302434","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21540484","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2O2Q","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2O2R","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3RHJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3RHL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3RHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3RHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3RHR","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 12 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"17302434","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"21540484","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2O2Q","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3RHJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3RHL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3RHO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3RHQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3RHR","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"21540484","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3RHO","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 12 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"O75891","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"UniProtKB","id":"Q8K009","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"UniProtKB","id":"Q8R0Y6","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"Q3SY69","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
