3REA
HIV-1 Nef protein in complex with engineered Hck-SH3 domain
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | Protein Nef | polymer | 166 | 19140.5 | 2 | UniProt (P03407) Pfam (PF00469) | HIV-1 M:B_ARV2/SF2 (HIV-1) | 3'ORF, Negative factor, F-protein, C-terminal core protein |
| 2 | B, D (B, D) | Tyrosine-protein kinase HCK | polymer | 61 | 7050.8 | 2 | UniProt (P08631) Pfam (PF00018) | Homo sapiens (human) | Hemopoietic cell kinase, p59-HCK/p60-HCK |
| 3 | E, F, G, H (A, B, C, D) | water | water | 18.0 | 331 | Chemie (HOH) |
Sequence modifications
A, C: 45 - 210 (UniProt: P03407)
B, D: 79 - 138 (UniProt: P08631)
| PDB | External Database | Details |
|---|---|---|
| Met 47 | Ile 47 | engineered mutation |
| Ala 48 | Thr 48 | engineered mutation |
| Ser 59 | Cys 59 | engineered mutation |
| Ala 210 | Cys 210 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met 78 | - | initiating methionine |
| Val 90 | Glu 90 | engineered mutation |
| Ser 91 | Ala 91 | engineered mutation |
| Trp 92 | Ile 92 | engineered mutation |
| Ser 93 | His 93 | engineered mutation |
| Pro 94 | His 94 | engineered mutation |
| Asp 95 | Glu 95 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 52382.6 | |
| All* | Total formula weight | 52382.6 |
