3REA
HIV-1 Nef protein in complex with engineered Hck-SH3 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 65.692, 65.692, 279.068 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.16989 |
Rwork | 0.168 |
R-free | 0.20544 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1efn |
RMSD bond length | 0.026 |
RMSD bond angle | 1.823 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC (5.5.0102) |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.770 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 40486 | |
<I/σ(I)> | 18.2 | |
Completeness [%] | 99.5 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 285 | 100 mM Tris buffer, 5% ethylne glycol, 10% PEG 8000, 0.2 M MgCl2, 15 mM MnCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K |