3RDE

Crystal structure of the catalytic domain of porcine leukocyte 12-lipoxygenase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Arachidonate 12-lipoxygenase, 12S-type	polymer	573	64766.2	4	UniProt (P16469) Pfam (PF00305) In PDB	Sus scrofa (pigs swine wild boar)	12S-LOX, 12S-lipoxygenase
2	A, D	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
3	A, B, C, D	FE (II) ION	non-polymer		55.8	4	Chemie (FE2)
4	A, B, C, D	3-{4-[(tridec-2-yn-1-yloxy)methyl]phenyl}propanoic acid	non-polymer		358.5	4	Chemie (OYP)
5		water	water		18.0	1274	Chemie (HOH)

Sequence modifications

A, B, C, D: 112 - 663 (UniProt: P16469)

PDB	External Database	Details
Met 91	-	initiating methionine
Gly 92	-	expression tag
Ser 93	-	expression tag
Ser 94	-	expression tag
His 95	-	expression tag
His 96	-	expression tag
His 97	-	expression tag
His 98	-	expression tag
His 99	-	expression tag
His 100	-	expression tag
Ser 101	-	expression tag
Ser 102	-	expression tag
Gly 103	-	expression tag
Leu 104	-	expression tag
Val 105	-	expression tag
Pro 106	-	expression tag
Arg 107	-	expression tag
Gly 108	-	expression tag
Ser 109	-	expression tag
His 110	-	expression tag
Met 111	-	expression tag
Ser 210	Cys 210	engineered mutation
Arg 218	Gln 218	engineered mutation
Ser 292	Cys 292	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	259064.9
Non-Polymers*	Number of molecules	10
	Total formula weight	1735.6
All*	Total formula weight	260800.5

*Water molecules are not included.