3RBB
HIV-1 NEF protein in complex with engineered HCK SH3 domain
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C | Protein Nef | polymer | 166 | 19140.5 | 2 | UniProt (P03407) Pfam (PF00469) In PDB | HIV-1 M:B_ARV2/SF2 (HIV-1) | 3'ORF, Negative factor, F-protein, C-terminal core protein |
2 | B, D | Tyrosine-protein kinase HCK | polymer | 61 | 7147.0 | 2 | UniProt (P08631) Pfam (PF00018) In PDB | Homo sapiens (human) | Hemopoietic cell kinase, p59-HCK/p60-HCK |
3 | A, C | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
4 | water | water | 18.0 | 208 | Chemie (HOH) |
Sequence modifications
A, C: 45 - 210 (UniProt: P03407)
B, D: 79 - 138 (UniProt: P08631)
PDB | External Database | Details |
---|---|---|
Met 47 | Ile 47 | engineered mutation |
Ala 48 | Thr 48 | engineered mutation |
Ser 59 | Cys 59 | engineered mutation |
Ala 210 | Cys 210 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met 78 | - | initiating methionine |
Tyr 90 | Glu 90 | engineered mutation |
Ser 91 | Ala 91 | engineered mutation |
Pro 92 | Ile 92 | engineered mutation |
Phe 93 | His 93 | engineered mutation |
Ser 94 | His 94 | engineered mutation |
Trp 95 | Glu 95 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 52574.9 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 124.1 | |
All* | Total formula weight | 52699.0 |