3RBB
HIV-1 NEF protein in complex with engineered HCK SH3 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.977032 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.190, 90.880, 169.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.990 - 2.350 |
R-factor | 0.20589 |
Rwork | 0.204 |
R-free | 0.24513 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1efn |
RMSD bond length | 0.014 |
RMSD bond angle | 1.329 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC (5.5.0109) |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 2.400 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 28573 | |
<I/σ(I)> | 22.18 | |
Completeness [%] | 99.5 | 98.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M Hepes, 8% ethylene glycol, 22% PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |