3Q22

X-ray crystal structure of the N4 mini-VRNAP and P2_7a promoter transcription initiation complex with GTP and Magnesium: substrate complex I

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	Virion RNA polymerase	polymer	1118	123193.5	2	UniProt (Q859P9) Pfam (PF21867) Pfam (PF21624) Pfam (PF21894) Pfam (PF21769)	Enterobacteria phage N4 (Bacteriophage N4)
2	B, D (C, D)	DNA (5'-D(*TP*GP*CP*CP*TP*CP*CP*CP*AP*GP*GP*CP*AP*TP*CP*CP*AP*AP*AP*AP*GP*AP*AP*GP*CP*GP*GP*AP*GP*CP*TP*TP*CP*TP*TP*C)-3')	polymer	36	11007.1	2
3	E, F, I, L (A, B, D)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	4	Chemie (GTP)
4	G, J (A, B)	DIHYDROGENPHOSPHATE ION	non-polymer		97.0	2	Chemie (2HP)
5	H, K (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	M, N, O, P (A, C, B, D)	water	water		18.0	1420	Chemie (HOH)

Sequence modifications

A, B: 1 - 1106 (UniProt: Q859P9)

PDB	External Database	Details
Met -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Arg -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	268401.2
Non-Polymers*	Number of molecules	8
	Total formula weight	2335.3
All*	Total formula weight	270736.5

*Water molecules are not included.