3Q22
X-ray crystal structure of the N4 mini-VRNAP and P2_7a promoter transcription initiation complex with GTP and Magnesium: substrate complex I
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Virion RNA polymerase | polymer | 1118 | 123193.5 | 2 | UniProt (Q859P9) Pfam (PF21867) Pfam (PF21624) Pfam (PF21894) Pfam (PF21769) In PDB | Enterobacteria phage N4 (Bacteriophage N4) | |
2 | C, D | DNA (5'-D(*TP*GP*CP*CP*TP*CP*CP*CP*AP*GP*GP*CP*AP*TP*CP*CP*AP*AP*AP*AP*GP*AP*AP*GP*CP*GP*GP*AP*GP*CP*TP*TP*CP*TP*TP*C)-3') | polymer | 36 | 11007.1 | 2 | |||
3 | A, B, D | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 4 | Chemie (GTP) | |||
4 | A, B | DIHYDROGENPHOSPHATE ION | non-polymer | 97.0 | 2 | Chemie (2HP) | |||
5 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
6 | water | water | 18.0 | 1420 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 1106 (UniProt: Q859P9)
PDB | External Database | Details |
---|---|---|
Met -11 | - | expression tag |
Gly -10 | - | expression tag |
Gly -9 | - | expression tag |
Ser -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
Arg -1 | - | expression tag |
Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 268401.2 | |
Non-Polymers* | Number of molecules | 8 |
Total formula weight | 2335.3 | |
All* | Total formula weight | 270736.5 |