3Q22

X-ray crystal structure of the N4 mini-VRNAP and P2_7a promoter transcription initiation complex with GTP and Magnesium: substrate complex I

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	Virion RNA polymerase	polymer	1118	123193.5	2	UniProt (Q859P9) Pfam (PF21867) Pfam (PF21624) Pfam (PF21894) Pfam (PF21769)	Enterobacteria phage N4 (Bacteriophage N4)
2	B, D (C, D)	DNA (5'-D(*TP*GP*CP*CP*TP*CP*CP*CP*AP*GP*GP*CP*AP*TP*CP*CP*AP*AP*AP*AP*GP*AP*AP*GP*CP*GP*GP*AP*GP*CP*TP*TP*CP*TP*TP*C)-3')	polymer	36	11007.1	2
3	E, F, I, L (A, B, D)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	4	Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466)
4	G, J (A, B)	DIHYDROGENPHOSPHATE ION	non-polymer		97.0	2	Chemie (2HP) PubChem (1003)
5	H, K (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG) PubChem (888)
6	M, N, O, P (A, C, B, D)	water	water		18.0	1420	Chemie (HOH)

Sequence modifications

A, B: 1 - 1106 (UniProt: Q859P9)

PDB	External Database	Details
Met -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Arg -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	268401.2
Non-Polymers*	Number of molecules	8
	Total formula weight	2335.3
All*	Total formula weight	270736.5

*Water molecules are not included.