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3Q22

X-ray crystal structure of the N4 mini-VRNAP and P2_7a promoter transcription initiation complex with GTP and Magnesium: substrate complex I

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X25
Synchrotron siteNSLS
BeamlineX25
Temperature [K]100
Detector technologyCCD
Collection date2005-07-30
DetectorADSC QUANTUM 315
Spacegroup nameP 21 21 21
Unit cell lengths83.300, 111.787, 276.850
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution50.000 - 2.110
R-factor0.209
Rwork0.208
R-free0.21900
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.008
RMSD bond angle1.117
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareCNS
Refinement softwareREFMAC (5.2.0019)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.180
High resolution limit [Å]2.1002.100
Rmerge0.0900.614
Number of reflections133457
<I/σ(I)>10.2
Completeness [%]89.773
Redundancy3.32.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP82940.1 M TRIS-HCL, 0.21 M DIBASIC AMMONIUM CITRATE, 0.09 M AMMONIUM DIHYDROGEN MONOPHOSPHATE, 25 % (DROP) 15% (RESERVOIR) PEG 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K
Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111TRIS-HCL
211DIBASIC AMMONIUM CITRATE
311AMMONIUM DIHYDROGEN MONOPHOSPHATE
411PEG 3350
512TRIS-HCL
612DIBASIC AMMONIUM CITRATE
712AMMONIUM DIHYDROGEN MONOPHOSPHATE
812PEG 3350

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PDB entries from 2024-11-13

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