3PY8
Crystal structure of a mutant of the large fragment of DNA polymerase I from thermus aquaticus in a closed ternary complex with DNA and ddCTP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase I | polymer | 540 | 60951.0 | 1 | UniProt (P19821) Pfam (PF09281) Pfam (PF00476) | Thermus aquaticus | Taq polymerase 1 |
| 2 | B (B) | DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(DOC))-3') | polymer | 12 | 3617.4 | 1 | |||
| 3 | C (C) | DNA (5'-D(*AP*A*AP*GP*GP*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3') | polymer | 16 | 4964.2 | 1 | |||
| 4 | D (A) | 2',3'-DIDEOXYCYTIDINE 5'-TRIPHOSPHATE | non-polymer | 451.2 | 1 | Chemie (DCT) | |||
| 5 | E, J (A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 6 | F, H, I, K, L (A, B, C) | MANGANESE (II) ION | non-polymer | 54.9 | 5 | Chemie (MN) | |||
| 7 | G (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 8 | M, N, O (A, B, C) | water | water | 18.0 | 523 | Chemie (HOH) |
Sequence modifications
A: 293 - 832 (UniProt: P19821)
| PDB | External Database | Details |
|---|---|---|
| Lys 614 | Ile 614 | engineered mutation |
| Lys 747 | Met 747 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 69532.6 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 934.3 | |
| All* | Total formula weight | 70466.9 |
