3PX4

Crystal Structure of Bacillus DNA Polymerase I Large Fragment Bound to DNA and ddCTP-dA Mismatch (wobble) in Ajar Conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	DNA polymerase I	polymer	592	67490.3	2	UniProt (Q5KWC1) Pfam (PF22619) Pfam (PF00476) In PDB	Geobacillus kaustophilus
2	B, E	DNA (5'-D(*CP*CP*TP*GP*AP*CP*TP*CP*(DOC))-3')	polymer	9	2635.8	2
3	C, F	DNA (5'-D(*CP*AP*TP*AP*GP*GP*AP*GP*TP*CP*AP*GP*G)-3')	polymer	13	4040.6	2
4	A, D	2',3'-DIDEOXYCYTIDINE 5'-TRIPHOSPHATE	non-polymer		451.2	2	Chemie (DCT)
5	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	D	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
7		water	water		18.0	1468	Chemie (HOH)

Sequence modifications

A, D: 285 - 876 (UniProt: Q5KWC1)

PDB	External Database	Details
Ala 598	Asp 600	engineered mutation
Tyr 710	Phe 712	engineered mutation
His 823	Arg 825	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	148333.4
Non-Polymers*	Number of molecules	5
	Total formula weight	1118.7
All*	Total formula weight	149452.1

*Water molecules are not included.