3PGF

Crystal structure of maltose bound MBP with a conformationally specific synthetic antigen binder (sAB)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Maltose-binding periplasmic protein	polymer	398	44175.6	1	UniProt (P0AEX9) Pfam (PF01547)	Escherichia coli	MBP,MMBP,Maltodextrin-binding protein
2	B (H)	SAB Heavy Chain	polymer	231	24669.4	1	UniProt (P0DOX5) Pfam (PF07686) Pfam (PF07654)	Homo sapiens (human)	Immunoglobulin gamma-1 heavy chain NIE
3	C (L)	SAB Light Chain	polymer	215	23330.9	1	UniProt (Q8TCD0) Pfam (PF07686) Pfam (PF07654)	Homo sapiens (human)
4	D (B)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
5	E, F (A)	IMIDAZOLE	non-polymer		69.1	2	Chemie (IMD)
6	G, H (H, L)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
7	I, J, K (A, H, L)	water	water		18.0	400	Chemie (HOH)

Sequence modifications

A: 1 - 367 (UniProt: P0AEX9)

PDB	External Database	Details
Met -30	-	initiating methionine
Lys -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Lys -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Lys -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Asn 367	Arg 393	conflict

H: -2 - 114 (PDB: 3PGF)
H: 115 - 221 (UniProt: P0DOX5)
L: 0 - 106 (PDB: 3PGF)
L: 107 - 214 (UniProt: Q8TCD0)

PDB	External Database	Details
Ser 123	Glu 148	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	92175.9
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	4
	Total formula weight	322.4
All*	Total formula weight	92840.6

*Water molecules are not included.