3PGF
Crystal structure of maltose bound MBP with a conformationally specific synthetic antigen binder (sAB)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Maltose-binding periplasmic protein | polymer | 398 | 44175.6 | 1 | UniProt (P0AEX9) Pfam (PF01547) In PDB | Escherichia coli | MBP,MMBP,Maltodextrin-binding protein |
2 | H | SAB Heavy Chain | polymer | 231 | 24669.4 | 1 | UniProt (P0DOX5) Pfam (PF07686) Pfam (PF07654) In PDB | Homo sapiens (human) | Immunoglobulin gamma-1 heavy chain NIE |
3 | L | SAB Light Chain | polymer | 215 | 23330.9 | 1 | UniProt (Q8TCD0) Pfam (PF07686) Pfam (PF07654) In PDB | Homo sapiens (human) | |
4 | B | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
5 | A | IMIDAZOLE | non-polymer | 69.1 | 2 | Chemie (IMD) | |||
6 | H, L | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
7 | water | water | 18.0 | 400 | Chemie (HOH) |
Sequence modifications
A: 1 - 367 (UniProt: P0AEX9)
H: -2 - 114 (PDB: 3PGF)
H: 115 - 221 (UniProt: P0DOX5)
L: 0 - 106 (PDB: 3PGF)
L: 107 - 214 (UniProt: Q8TCD0)
PDB | External Database | Details |
---|---|---|
Met -30 | - | initiating methionine |
Lys -29 | - | expression tag |
His -28 | - | expression tag |
His -27 | - | expression tag |
His -26 | - | expression tag |
His -25 | - | expression tag |
His -24 | - | expression tag |
His -23 | - | expression tag |
His -22 | - | expression tag |
His -21 | - | expression tag |
His -20 | - | expression tag |
His -19 | - | expression tag |
Ser -18 | - | expression tag |
Ser -17 | - | expression tag |
Asp -16 | - | expression tag |
Tyr -15 | - | expression tag |
Lys -14 | - | expression tag |
Asp -13 | - | expression tag |
Asp -12 | - | expression tag |
Asp -11 | - | expression tag |
Asp -10 | - | expression tag |
Lys -9 | - | expression tag |
Gly -8 | - | expression tag |
Glu -7 | - | expression tag |
Asn -6 | - | expression tag |
Leu -5 | - | expression tag |
Tyr -4 | - | expression tag |
Phe -3 | - | expression tag |
Gln -2 | - | expression tag |
Gly -1 | - | expression tag |
Ser 0 | - | expression tag |
Asn 367 | Arg 393 | conflict |
H: 115 - 221 (UniProt: P0DOX5)
L: 0 - 106 (PDB: 3PGF)
L: 107 - 214 (UniProt: Q8TCD0)
PDB | External Database | Details |
---|---|---|
Ser 123 | Glu 148 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 92175.9 | |
Branched | Number of molecules | 1 |
Total formula weight | 342.3 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 322.4 | |
All* | Total formula weight | 92840.6 |