3PB1

Crystal Structure of a Michaelis Complex between Plasminogen Activator Inhibitor-1 and Urokinase-type Plasminogen Activator

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (I)	Plasminogen activator inhibitor 1	polymer	379	42795.1	1	UniProt (P05121) Pfam (PF00079)	Homo sapiens (human)	PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1
2	B (E)	Plasminogen activator, urokinase	polymer	253	28426.4	1	UniProt (Q5SWW8) Pfam (PF00089) UniProt (by SIFTS) (P00749)	Homo sapiens (human)
3	C, D (E)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	E, F (I, E)	water	water		18.0	314	Chemie (HOH)

Sequence modifications

I: 1 - 379 (UniProt: P05121)

PDB	External Database	Details
His 150	Asn 173	engineered mutation
Thr 154	Lys 177	engineered mutation
Gln 158	Asp 181	conflict
Leu 319	Gln 342	engineered mutation
Ile 354	Met 377	engineered mutation

E: 16 - 250 (UniProt: Q5SWW8)

PDB	External Database	Details
Ala 122	Cys 263	conflict
Gln 145	Asn 286	conflict
Ala 195	Ser 340	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	71221.4
Non-Polymers*	Number of molecules	2
	Total formula weight	192.1
All*	Total formula weight	71413.6

*Water molecules are not included.