3P8B

X-ray crystal structure of Pyrococcus furiosus transcription elongation factor Spt4/5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	DNA-directed RNA polymerase, subunit e''	polymer	81	9136.4	2	UniProt (Q8U440) Pfam (PF06093)	Pyrococcus furiosus
2	B, D (B, D)	Transcription antitermination protein nusG	polymer	152	16816.6	2	UniProt (Q8TZK1) Pfam (PF03439) Pfam (PF00467)	Pyrococcus furiosus
3	E, M (A, C)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	F, J, K, L, P... (A, B, D)	BETA-MERCAPTOETHANOL	non-polymer		78.1	6	Chemie (BME)
5	G, H, I, N, O (B, D)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
6	R, S, T, U (A, B, C, D)	water	water		18.0	111	Chemie (HOH)

Sequence modifications

A, C: 1 - 61 (UniProt: Q8U440)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	51906.0
Non-Polymers*	Number of molecules	13
	Total formula weight	1060.1
All*	Total formula weight	52966.1

*Water molecules are not included.