3P8B
X-ray crystal structure of Pyrococcus furiosus transcription elongation factor Spt4/5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Detector technology | CCD |
Collection date | 2009-11-09 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9181 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 40.532, 87.213, 133.107 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.23928 |
Rwork | 0.236 |
R-free | 0.29234 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 1.991 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.800 |
Number of reflections | 44007 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 0.1 M Bis-Tris (pH 5.5), 0.2 M NaCl, and 25% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K |