3P6Z

Structural basis of thrombin mediated factor V activation: essential role of the hirudin-like sequence Glu666-Glu672 for processing at the heavy chain-B domain junction

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, G	Thrombin light chain	polymer	36	4096.5	2	UniProt (P00734) Pfam (PF09396) In PDB	Homo sapiens (human)	Coagulation factor II, Thrombin light chain
2	B, H	Thrombin heavy chain	polymer	259	29780.2	2	UniProt (P00734) Pfam (PF00089) In PDB	Homo sapiens (human)	Coagulation factor II, Thrombin heavy chain
3	C, I	Coagulation factor V	polymer	71	8391.9	2	UniProt (P12259) In PDB	Homo sapiens (human)	Activated protein C cofactor
4	A, B, G, H	SODIUM ION	non-polymer		23.0	9	Chemie (NA)
5	B, H	D-phenylalanyl-N-[(2S,3S)-6-{[amino(iminio)methyl]amino}-1-chloro-2-hydroxyhexan-3-yl]-L-prolinamide	non-polymer		454.0	2	BIRD (PRD_000020) Chemie (0G6)
6	B, H	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
7	B	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
8	B	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	1	Chemie (MPD)
9		water	water		18.0	505	Chemie (HOH)

Sequence modifications

C, I: 657 - 709 (UniProt: P12259)

PDB	External Database	Details
Ala 639	-	expression tag
His 640	-	expression tag
His 641	-	expression tag
His 642	-	expression tag
His 643	-	expression tag
His 644	-	expression tag
His 645	-	expression tag
Val 646	-	expression tag
Gly 647	-	expression tag
Thr 648	-	expression tag
Trp 649	-	expression tag
Glu 650	-	expression tag
Asn 651	-	expression tag
Leu 652	-	expression tag
Tyr 653	-	expression tag
Phe 654	-	expression tag
Gln 655	-	expression tag
Ser 656	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	84537.4
Non-Polymers*	Number of molecules	17
	Total formula weight	1781.8
All*	Total formula weight	86319.2

*Water molecules are not included.