3P0G

Structure of a nanobody-stabilized active state of the beta2 adrenoceptor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Beta-2 adrenergic receptor, Lysozyme	polymer	501	56716.5	1	UniProt (P07550) UniProt (P00720) Pfam (PF00001) Pfam (PF00959)	Homo sapiens (human Bacteriophage T4)	Endolysin, Lysis protein, Muramidase
2	B (B)	Camelid Antibody Fragment	polymer	126	13784.2	1		LAMA GLAMA
3	C (A)	8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one	non-polymer		370.4	1	Chemie (P0G)

Sequence modifications

A: 1 - 227 (UniProt: P07550)

PDB	External Database	Details
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Ala 0	-	expression tag
Arg 16	Gly 16	SEE REMARK 999
Gln 27	Glu 27	SEE REMARK 999
Glu 187	Asn 187	engineered mutation

A: 227 - 233 (UniProt: P00720)

PDB	External Database	Details
Thr 229	Cys 54	conflict
Ala 230	Cys 97	conflict

A: 233 - 365 (UniProt: P07550)
B: 1 - 120 (PDB: 3P0G)

PDB	External Database	Details
His 121	-	expression tag
His 122	-	expression tag
His 123	-	expression tag
His 124	-	expression tag
His 125	-	expression tag
His 126	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	70500.7
Non-Polymers*	Number of molecules	1
	Total formula weight	370.4
All*	Total formula weight	70871.1

*Water molecules are not included.