3P0G
Structure of a nanobody-stabilized active state of the beta2 adrenoceptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 78 |
Detector technology | CCD |
Collection date | 2010-03-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 236.686, 45.660, 71.375 |
Unit cell angles | 90.00, 102.34, 90.00 |
Refinement procedure
Resolution | 37.000 - 3.500 |
R-factor | 0.2428 |
Rwork | 0.235 |
R-free | 0.30830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 2RH1 and 3DWT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.220 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.560 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.200 | |
Number of reflections | 9308 | |
<I/σ(I)> | 4.9 | |
Completeness [%] | 94.8 | 93.7 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 293 | 36-44% PEG 400, 100 mM Tris pH 8.0, 4% DMSO, 1% 1,2,3-heptanetriol, twin-syringe mixing method, temperature 293K | |
1 | 8 | 293 | 36-44% PEG 400, 100 mM Tris pH 8.0, 4% DMSO, 1% 1,2,3-heptanetriol, twin-syringe mixing method, temperature 293K |