3OY9

Crystal structure of the Prototype Foamy Virus (PFV) intasome in complex with manganese at 2.55 resolution

Replaces:  3L2S

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	PFV integrase	polymer	395	44456.7	2	UniProt (P14350) Pfam (PF17921) Pfam (PF00665) Pfam (PF18103)	Human spumaretrovirus (SFVcpz(hu))	p42In
2	C (C)	DNA (5'-D(*AP*TP*TP*GP*TP*CP*AP*TP*GP*GP*AP*AP*TP*TP*TP*CP*GP*CP*A)-3')	polymer	19	5834.8	1
3	D (D)	DNA (5'-D(*TP*GP*CP*GP*AP*AP*AP*TP*TP*CP*CP*AP*TP*GP*AP*CP*A)-3')	polymer	17	5195.4	1
4	E (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	F, G, N (A, B)	MANGANESE (II) ION	non-polymer		54.9	3	Chemie (MN)
6	H, O (A, B)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
7	I, J, K, L (A)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
8	M (A)	AMMONIUM ION	non-polymer		18.0	1	Chemie (NH4)
9	P, Q, R, S (A, B, C, D)	water	water		18.0	274	Chemie (HOH)

Sequence modifications

A, B: 1 - 392 (UniProt: P14350)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag
Ser 217	Gly 968	variant
Gly 218	Ser 969	variant

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	99943.6
Non-Polymers*	Number of molecules	11
	Total formula weight	808.8
All*	Total formula weight	100752.3

*Water molecules are not included.