3OTK

Structure and mechanisim of core 2 beta1,6-n-acetylglucosaminyltransferase: a Metal-ion independent gt-a glycosyltransferase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase	polymer	391	45303.9	4	UniProt (Q09324) Pfam (PF02485)	Mus musculus (mouse)	Core 2-branching enzyme, Core2-GlcNAc-transferase, C2GNT
2	E, F (E, F)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	2	In PDB GlyTouCan (G21290RB)
3	G, H (A)	HEPTANE-1,2,3-TRIOL	non-polymer		148.2	2	Chemie (HTO)
4	I, N (A, C)	SODIUM ION	non-polymer		23.0	2	Chemie (NA) PubChem (923)
5	J, L, O, Q (A, B, C, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
6	K, M, P, R (A, B, C, D)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	4	Chemie (UDP) PubChem (25323044) PubChem (25323048) PubChem (44298965) PubChem (57448939) PubChem (53486216) PubChem (60098857) PubChem (91746160) PubChem (92332560) PubChem (92332561) PubChem (92332562) PubChem (92332563) PubChem (93088131) PubChem (93630083) PubChem (1158) PubChem (6031) PubChem (3084486) PubChem (6101632) PubChem (6604175) PubChem (11811538) PubChem (12049241)
7	S, T, U, V (A, B, C, D)	water	water		18.0	533	Chemie (HOH)

Sequence modifications

A, B, C, D: 38 - 428 (UniProt: Q09324)

PDB	External Database	Details
Ser 217	Cys 217	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	181215.5
Branched	Number of molecules	2
	Total formula weight	1141.1
Non-Polymers*	Number of molecules	12
	Total formula weight	2843.9
All*	Total formula weight	185200.4

*Water molecules are not included.