3OTK
Structure and mechanisim of core 2 beta1,6-n-acetylglucosaminyltransferase: a Metal-ion independent gt-a glycosyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9771 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.874, 101.020, 136.612 |
| Unit cell angles | 90.00, 93.42, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.16806 |
| Rwork | 0.165 |
| R-free | 0.21926 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.172 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.200 | 2.210 |
| Rmerge | 0.134 | 0.587 |
| Number of reflections | 93761 | |
| <I/σ(I)> | 10.6 | 2.1 |
| Completeness [%] | 93.2 | 74.6 |
| Redundancy | 3.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 296 | 22% polyethylene glycol 4000, 0.15 M glycine pH 9.0, 0.6 M LiCl, 1.5% 1,2,3 heptanetriol, 25 mM UDP-GlcNAc, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
