3OLA

Poliovirus polymerase elongation complex with 2'-deoxy-CTP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I, M (A, E, I, M)	Polymerase	polymer	471	53761.2	4	UniProt (B3VQP5) Pfam (PF00680)	Human poliovirus 1
2	B, F, J, N (B, F, J, N)	RNA (5'-R(*AP*AP*GP*UP*CP*UP*CP*CP*AP*GP*GP*UP*CP*UP*CP*UP*CP*GP*UP*CP*CP*GP*GP*AP*AP*A)-3')	polymer	26	8280.0	4
3	C, G (C, G)	DNA/RNA (5'-R(*GP*CP*CP*CP*GP*GP*AP*CP*GP*AP*GP*AP*GP*A)-D(P*C)-3')	polymer	15	4853.0	2
4	D, H, L, P (D, H, L, P)	RNA (5'-R(*GP*GP*GP*AP*GP*AP*UP*GP*A)-3')	polymer	9	2974.9	4
5	K, O (K, O)	RNA (5'-R(*GP*CP*CP*CP*GP*GP*AP*CP*GP*AP*GP*AP*GP*A)-3')	polymer	14	4563.8	2
6	BA, HA, Q, X (I, M, A, E)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
7	AA, CA, DA, IA, JA... (E, I, M, A)	ISOPROPYL ALCOHOL	non-polymer		60.1	10	Chemie (IPA)
8	EA, U, V (I, A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
9	FA, W (I, A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
10	GA, KA (K, O)	2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE	non-polymer		467.2	2	Chemie (DCP)
11	LA, MA, NA, OA, PA... (A, B, C, D, E...)	water	water		18.0	503	Chemie (HOH)

Sequence modifications

A, E, I, M: 1 - 461 (UniProt: B3VQP5)

PDB	External Database	Details
Asp 446	Leu 2194	engineered mutation
Gly 462	-	expression tag
Ser 463	-	expression tag
Ser 464	-	expression tag
Ser 465	-	expression tag
His 466	-	expression tag
His 467	-	expression tag
His 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag
His 471	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	278897.7
Non-Polymers*	Number of molecules	21
	Total formula weight	2299.4
All*	Total formula weight	281197.2

*Water molecules are not included.