3OL7

Poliovirus polymerase elongation complex with CTP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I, M (A, E, I, M)	Polymerase	polymer	471	53761.2	4	UniProt (B3VQP5) Pfam (PF00680) UniProt (by SIFTS) (P03300)	Human poliovirus 1
2	B, F, J, N (B, F, J, N)	RNA (5'-R(*AP*AP*GP*UP*CP*UP*CP*CP*AP*GP*GP*UP*CP*UP*CP*UP*CP*GP*UP*CP*CP*GP*GP*AP*AP*A)-3')	polymer	26	8280.0	4
3	C, G, K, O (C, G, K, O)	RNA (5'-R(*GP*CP*CP*CP*GP*GP*AP*CP*GP*AP*GP*AP*GP*AP*C)-3')	polymer	15	4869.0	4
4	D, H, L, P (D, H, L, P)	RNA (5'-R(*GP*GP*GP*AP*GP*AP*UP*GP*A)-3')	polymer	9	2974.9	4
5	AA, JA, Q, R, UA... (E, I, A, M)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
6	BA, S, TA, VA (E, A, K, M)	PYROPHOSPHATE 2-	non-polymer		176.0	4	Chemie (POP)
7	BB, CA, DA, EA, EB... (N, E, O, I, A...)	ISOPROPYL ALCOHOL	non-polymer		60.1	15	Chemie (IPA)
8	CB, GA, RA, X (N, E, J, A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	4	Chemie (PEG)
9	AB, IA, QA, Y (M, E, I, A)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
10	DB, HA, MA, NA, OA... (N, E, I, J, M)	GLYCEROL	non-polymer		92.1	8	Chemie (GOL)
11	FB, GB, HB, IB, JB... (A, B, C, D, E...)	water	water		18.0	549	Chemie (HOH)

Sequence modifications

A, E, I, M: 1 - 461 (UniProt: B3VQP5)

PDB	External Database	Details
Asp 446	Leu 2194	engineered mutation
Gly 462	-	expression tag
Ser 463	-	expression tag
Ser 464	-	expression tag
Ser 465	-	expression tag
His 466	-	expression tag
His 467	-	expression tag
His 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag
His 471	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	279540.1
Non-Polymers*	Number of molecules	41
	Total formula weight	3174.0
All*	Total formula weight	282714.0

*Water molecules are not included.