3O78

The structure of Ca2+ Sensor (Case-12)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Myosin light chain kinase, smooth muscle,Green fluorescent protein,Green fluorescent protein,Calmodulin-1	polymer	417	46491.9	2	UniProt (P11799) UniProt (P42212) UniProt (P0DP23) Pfam (PF01353) Pfam (PF13499) In PDB	Gallus gallus (Chicken)	MLCK,Telokin
2	A, B	CALCIUM ION	non-polymer		40.1	8	Chemie (CA)
3		water	water		18.0	77	Chemie (HOH)

Sequence modifications

A, B: 5 - 24 (UniProt: P11799)

PDB	External Database	Details
Gly 1	-	expression tag
Pro 2	-	expression tag
Gly 3	-	expression tag
Ser 4	-	expression tag
Ser 5	Ala 1730	engineered mutation
Asn 10	Gln 1735	engineered mutation

A, B: 25 - 116 (UniProt: P42212)

PDB	External Database	Details
Leu 25	Ser 147	engineered mutation
Glu 26	His 148	engineered mutation
Ala 41	Val 163	engineered mutation
Val 49	Ile 171	engineered mutation
Phe 81	Thr 203	engineered mutation
Val 84	Ala 206	engineered mutation
Leu 109	His 231	engineered mutation
Asn 116	Lys 238	engineered mutation

A, B: 126 - 270 (UniProt: P42212)

PDB	External Database	Details
Val 117	-	linker
Asp 118	-	linker
Gly 119	-	linker
Gly 120	-	linker
Ser 121	-	linker
Gly 122	-	linker
Gly 123	-	linker
Thr 124	-	linker
Gly 125	-	linker
Leu 170	Phe 46	engineered mutation
Leu 188	Phe 64	engineered mutation
Cr2 190	Ser 65	chromophore
Cr2 190	Tyr 66	chromophore
Cr2 190	Gly 67	chromophore
Leu 192	Val 68	engineered mutation
Lys 193	Gln 69	engineered mutation
Ala 196	Ser 72	see sequence details
Gly 253	Asp 129	engineered mutation
Thr 269	Tyr 145	engineered mutation
Arg 270	Asn 146	engineered mutation

A, B: 271 - 417 (UniProt: P0DP23)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	92983.8
Non-Polymers*	Number of molecules	8
	Total formula weight	320.6
All*	Total formula weight	93304.4

*Water molecules are not included.