3O78
The structure of Ca2+ Sensor (Case-12)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 77 |
Detector technology | IMAGE PLATE |
Collection date | 2007-08-20 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.374, 101.635, 82.211 |
Unit cell angles | 90.00, 91.45, 90.00 |
Refinement procedure
Resolution | 50.830 - 2.600 |
R-factor | 0.24546 |
Rwork | 0.242 |
R-free | 0.31614 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o77 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.726 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.710 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 23464 | |
<I/σ(I)> | 12 | 2.8 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | Reservoir: 100mM Tris HCL (pH 5.5), 100mM (NH4)2SO4, 21% PEG 3350; Protein stock: 7.6 mg/ml Protein, 50mM Tris HCl (pH 7.4), 150mM NaCl; Seed stock solution: 20mM CaCl2, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |