3NDH

Restriction endonuclease in complex with substrate DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	restriction endonuclease THAI	polymer	225	26417.4	2	UniProt (Q9HJY3) Pfam (PF15514) In PDB	Thermoplasma acidophilum	Putative uncharacterized protein Ta0828
2	C	DNA (5'-D(*G*GP*TP*AP*CP*GP*CP*GP*AP*TP*G)-3')	polymer	11	3414.2	1
3	D	DNA (5'-D(*C*CP*AP*TP*CP*GP*CP*GP*TP*AP*C)-3')	polymer	11	3294.2	1
4	A, B	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
5	A, B	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
6	A, B	TRIETHYLENE GLYCOL	non-polymer		150.2	3	Chemie (PGE)
7	A, B, C	ACETATE ION	non-polymer		59.0	4	Chemie (ACT)
8	A	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
9		water	water		18.0	470	Chemie (HOH)

Sequence modifications

A, B: 2 - 216 (UniProt: Q9HJY3)

PDB	External Database	Details
Met -8	-	expression tag
Gly -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Glu 0	-	expression tag
Phe 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	59543.1
Non-Polymers*	Number of molecules	13
	Total formula weight	995.7
All*	Total formula weight	60538.8

*Water molecules are not included.