3NDH
Restriction endonuclease in complex with substrate DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.310, 72.910, 88.760 |
| Unit cell angles | 90.00, 101.99, 90.00 |
Refinement procedure
| Resolution | 19.510 - 1.300 |
| R-factor | 0.1723 |
| Rwork | 0.172 |
| R-free | 0.18318 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.261 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.074 | 0.266 |
| Number of reflections | 118539 | |
| <I/σ(I)> | 5 | 2.8 |
| Completeness [%] | 98.7 | 99.4 |
| Redundancy | 2.8 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 294 | 0.1M sodium acetate PH 4.5, 200MM, ammonium sulfate, 30% PEG400, 1.5% 1,2,3-heptanetriol, VAPOR, DIFFUSION, SITTING DROP, TEMPERATURE 294K, VAPOR DIFFUSION, HANGING DROP |
