3N3T

Crystal structure of putative diguanylate cyclase/phosphodiesterase complex with cyclic di-gmp

Replaces:  3II8

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	PUTATIVE DIGUANYLATE CYCLASE/PHOSPHODIESTERASE	polymer	294	32876.1	2	UniProt (Q3SJE6) Pfam (PF00563)	THIOBACILLUS DENITRIFICANS
2	C, G (A, B)	9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one)	non-polymer		690.4	2	Chemie (C2E)
3	D, E, H, I (A, B)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4	F (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5	J, K (A, B)	water	water		18.0	225	Chemie (HOH)

Sequence modifications

A, B: 487 - 758 (UniProt: Q3SJE6)

PDB	External Database	Details
Mse 465	-	expression tag
Gly 466	-	expression tag
Ser 467	-	expression tag
Ser 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag
His 471	-	expression tag
His 472	-	expression tag
His 473	-	expression tag
His 474	-	expression tag
Ser 475	-	expression tag
Ser 476	-	expression tag
Gly 477	-	expression tag
Arg 478	-	expression tag
Glu 479	-	expression tag
Asn 480	-	expression tag
Leu 481	-	expression tag
Tyr 482	-	expression tag
Phe 483	-	expression tag
Gln 484	-	expression tag
Gly 485	-	expression tag
His 486	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	65752.3
Non-Polymers*	Number of molecules	7
	Total formula weight	1513.5
All*	Total formula weight	67265.8

*Water molecules are not included.