3N3T
Crystal structure of putative diguanylate cyclase/phosphodiesterase complex with cyclic di-gmp
Replaces: 3II8Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-08 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.560, 63.201, 173.987 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.955 - 2.350 |
| R-factor | 0.18757 |
| Rwork | 0.184 |
| R-free | 0.25363 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.537 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.370 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.100 | 0.459 |
| Number of reflections | 24433 | |
| <I/σ(I)> | 34.6 | 5.19 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.9 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.2 M MAGNESIUM CHLORIDE, 25% PEG3350, 0.1M BIS-TRIS, 2.5MM CYCLIC DI-GMP, PH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
