3MVB

Crystal structure of a triple RFY mutant of human MTERF1 bound to the termination sequence

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (O)	Transcription termination factor, mitochondrial	polymer	343	39070.3	1	UniProt (Q99551) Pfam (PF02536)	Homo sapiens (human)	Mitochondrial transcription termination factor 1, mTERF
2	B (D)	5'-D(*AP*TP*TP*AP*CP*CP*GP*GP*GP*CP*TP*CP*TP*GP*CP*CP*AP*TP*CP*TP*TP*A)-3'	polymer	22	6678.3	1
3	C (E)	5'-D(*TP*AP*AP*GP*AP*TP*GP*GP*CP*AP*GP*AP*GP*CP*CP*CP*GP*GP*TP*AP*AP*T)-3'	polymer	22	6825.4	1
4	D, E, F (O, D, E)	water	water		18.0	38	Chemie (HOH)

Sequence modifications

O: 57 - 396 (UniProt: Q99551)

PDB	External Database	Details
Gly 54	-	expression tag
His 55	-	expression tag
Met 56	-	expression tag
Ala 162	Arg 162	engineered mutation
Ala 243	Phe 243	engineered mutation
Ala 288	Tyr 288	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	52574.0
All*	Total formula weight	52574.0

*Water molecules are not included.