3MVB
Crystal structure of a triple RFY mutant of human MTERF1 bound to the termination sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-21 |
| Detector | ADSC |
| Wavelength(s) | 1.09 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 87.526, 91.431, 159.047 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.762 - 2.786 |
| R-factor | 0.206 |
| Rwork | 0.202 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.274 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
| High resolution limit [Å] | 2.786 | 7.590 | 2.786 |
| Rmerge | 0.091 | 0.023 | 0.661 |
| Number of reflections | 16183 | ||
| <I/σ(I)> | 9.7 | ||
| Completeness [%] | 99.4 | 99.3 | 99.1 |
| Redundancy | 5.8 | 5.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | PEG 3350, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
