3MHS

Structure of the SAGA Ubp8/Sgf11/Sus1/Sgf73 DUB module bound to ubiquitin aldehyde

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ubiquitin carboxyl-terminal hydrolase 8	polymer	476	54033.6	1	UniProt (P50102) Pfam (PF02148) Pfam (PF00443) In PDB	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	Ubiquitin thioesterase 8, Ubiquitin-specific-processing protease 8, Deubiquitinating enzyme 8
2	B	Protein SUS1	polymer	96	11094.5	1	UniProt (Q6WNK7) Pfam (PF10163) In PDB	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)
3	C	SAGA-associated factor 11	polymer	99	11297.6	1	UniProt (Q03067) Pfam (PF18519) Pfam (PF08209) In PDB	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	11 kDa SAGA-associated factor
4	D	Ubiquitin	polymer	76	8560.8	1	UniProt (P62988) Pfam (PF00240) UniProt (by SIFTS) (P0CG48) In PDB	Homo sapiens (human)
5	E	SAGA-associated factor 73	polymer	96	10824.3	1	UniProt (P53165) Pfam (PF18508) In PDB	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	73 kDa SAGA-associated factor, SAGA histone acetyltransferase complex 73 kDa subunit
6	A	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
7	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
8	A, C, E	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
9		water	water		18.0	845	Chemie (HOH)

Sequence modifications

A: 1 - 471 (UniProt: P50102)

PDB	External Database	Details
Gly -4	-	expression tag
Ala -3	-	expression tag
Ala -2	-	expression tag
Ala -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	95810.9
Non-Polymers*	Number of molecules	10
	Total formula weight	677.4
All*	Total formula weight	96488.3

*Water molecules are not included.