3MHS
Structure of the SAGA Ubp8/Sgf11/Sus1/Sgf73 DUB module bound to ubiquitin aldehyde
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.94944 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.600, 102.084, 120.903 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.100 - 1.890 |
| R-factor | 0.16148 |
| Rwork | 0.159 |
| R-free | 0.20757 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.399 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.100 | 1.930 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.096 | 0.886 |
| Number of reflections | 73928 | |
| <I/σ(I)> | 29.5 | 2.25 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 13.7 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Hepes pH 6.5, 10% (w/v) PEG 8000, 20% (v/v) Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
