3L8B

Crystal structure of a replicative DNA polymerase bound to the oxidized guanine lesion guanidinohydantoin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA polymerase	polymer	906	105069.6	2	UniProt (Q38087) Pfam (PF03104) Pfam (PF00136)	Enterobacteria phage RB69 (Bacteriophage RB69)	Gp43
2	C, E (C, E)	DNA (5'-D(*AP*C*TP*(G35)P*TP*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*GP*CP*G)-3')	polymer	18	5522.6	2
3	D, F (D, F)	DNA (5'-D(*GP*CP*GP*GP*AP*CP*TP*GP*CP*TP*TP*AP*A)-3')	polymer	13	3991.6	2
4	G, H (B)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
5	I, J, K, L, M... (A, B, C, D, E...)	water	water		18.0	998	Chemie (HOH)

Sequence modifications

A, B: 1 - 903 (UniProt: Q38087)

PDB	External Database	Details
Ala 222	Asp 222	engineered mutation
Ala 327	Asp 327	engineered mutation
His 904	-	expression tag
His 905	-	expression tag
His 906	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	229167.6
Non-Polymers*	Number of molecules	2
	Total formula weight	192.1
All*	Total formula weight	229359.7

*Water molecules are not included.