3L8B
Crystal structure of a replicative DNA polymerase bound to the oxidized guanine lesion guanidinohydantoin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-23 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03320 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.276, 120.246, 136.963 |
Unit cell angles | 90.00, 95.96, 90.00 |
Refinement procedure
Resolution | 48.810 - 2.150 |
Rwork | 0.219 |
R-free | 0.25450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ig9 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.208 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.21) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.810 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.126 | 0.707 |
Number of reflections | 239932 | |
<I/σ(I)> | 9.9 | 2 |
Completeness [%] | 88.8 | 99.5 |
Redundancy | 4.3 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | reservoir: 12% PEG2000MME, 100mM Sodium Acetate, 150mM Magnesium Sulfate, 100mM Hepes pH 7.0, 6% Glycerol and 2mM Beta-mercaptoethanol, VAPOR DIFFUSION, HANGING DROP, temperature 297K |