3K0J
Crystal structure of the E. coli ThiM riboswitch in complex with thiamine pyrophosphate and the U1A crystallization module
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | U1 small nuclear ribonucleoprotein A | polymer | 96 | 11079.9 | 4 | UniProt (P09012) Pfam (PF00076) | Homo sapiens (human) | U1 snRNP protein A, U1A protein, U1-A |
| 2 | E, F (E, F) | RNA (87-MER) | polymer | 87 | 28019.7 | 2 | |||
| 3 | G, N (E, F) | THIAMINE DIPHOSPHATE | non-polymer | 425.3 | 2 | Chemie (TPP) | |||
| 4 | H, I, J, K, L... (E, F) | MAGNESIUM ION | non-polymer | 24.3 | 8 | Chemie (MG) |
Sequence modifications
A, B, C, D: 202 - 297 (UniProt: P09012)
| PDB | External Database | Details |
|---|---|---|
| His 231 | Tyr 31 | engineered mutation |
| Arg 236 | Gln 36 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 100359.1 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1045.1 | |
| All* | Total formula weight | 101404.2 |
