3JWQ

Crystal structure of chimeric PDE5/PDE6 catalytic domain complexed with sildenafil

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	cGMP-specific 3',5'-cyclic phosphodiesterase catalytic domain, Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha chimera	polymer	330	38282.1	4	UniProt (O76074) UniProt (P51160) Pfam (PF00233)	Homo sapiens (human)	cGMP-binding cGMP-specific phosphodiesterase, CGB-PDE, cGMP phosphodiesterase 6C
2	E, H, K, N (A, B, C, D)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
3	F, I, L, O (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4	G, J, M, P (A, B, C, D)	5-{2-ETHOXY-5-[(4-METHYLPIPERAZIN-1-YL)SULFONYL]PHENYL}-1-METHYL-3-PROPYL-1H,6H,7H-PYRAZOLO[4,3-D]PYRIMIDIN-7-ONE	non-polymer		474.6	4	Chemie (VIA)
5	Q, R, S, T (A, B, C, D)	water	water		18.0	13	Chemie (HOH)

Sequence modifications

A, B, C, D: 535 - 786 (UniProt: O76074)

PDB	External Database	Details
Gly 531	-	expression tag
Ser 532	-	expression tag
His 533	-	expression tag
Met 534	-	expression tag

A, B, C, D: 787 - 826 (UniProt: P51160)
A, B, C, D: 827 - 860 (UniProt: O76074)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	153128.3
Non-Polymers*	Number of molecules	12
	Total formula weight	2257.2
All*	Total formula weight	155385.5

*Water molecules are not included.