3JCM
Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Pre-mRNA-splicing factor 8 | polymer | 2413 | 279867.5 | 1 | UniProt (P33334) Pfam (PF08082) Pfam (PF08083) Pfam (PF10598) Pfam (PF10597) Pfam (PF10596) Pfam (PF12134) Pfam (PF08084) | Saccharomyces cerevisiae S288c | |
| 2 | B (B) | U4/U6 small nuclear ribonucleoprotein PRP4 | polymer | 465 | 52507.0 | 1 | UniProt (P20053) Pfam (PF00400) | Saccharomyces cerevisiae S288c | Pre-mRNA-processing protein 4 |
| 3 | C (I) | Pre-mRNA-processing factor 31 | polymer | 494 | 56382.5 | 1 | UniProt (P49704) Pfam (PF01798) Pfam (PF09785) | Saccharomyces cerevisiae S288c | |
| 4 | D (G) | Pre-mRNA-splicing factor 6 | polymer | 899 | 104370.1 | 1 | UniProt (P19735) Pfam (PF06424) | Saccharomyces cerevisiae S288c | |
| 5 | E (K) | U4/U6 small nuclear ribonucleoprotein PRP3 | polymer | 469 | 55974.3 | 1 | UniProt (Q03338) Pfam (PF08572) Pfam (PF06544) | Saccharomyces cerevisiae S288c | Pre-mRNA-splicing factor 3 |
| 6 | F (L) | Spliceosomal protein DIB1 | polymer | 143 | 16798.4 | 1 | UniProt (Q06819) Pfam (PF02966) | Saccharomyces cerevisiae S288c | |
| 7 | G (M) | 13 kDa ribonucleoprotein-associated protein | polymer | 126 | 13582.9 | 1 | UniProt (P39990) Pfam (PF01248) | Saccharomyces cerevisiae S288c | Small nuclear ribonucleoprotein-associated protein 1 |
| 8 | H (H) | Pre-mRNA-splicing factor SNU114 | polymer | 1008 | 114174.0 | 1 | UniProt (P36048) Pfam (PF16004) Pfam (PF00009) Pfam (PF03764) Pfam (PF00679) | Saccharomyces cerevisiae S288c | 114 kDa U5 small nuclear ribonucleoprotein component, Growth inhibitory protein 10 |
| 9 | I (N) | Pre-mRNA-splicing helicase BRR2 | polymer | 2163 | 246470.3 | 1 | UniProt (P32639) Pfam (PF21188) Pfam (PF18149) Pfam (PF00270) Pfam (PF00271) Pfam (PF23445) Pfam (PF02889) | Saccharomyces cerevisiae S288c | Protein Snu246 |
| 10 | J, Q (R, J) | Small nuclear ribonucleoprotein Sm D3 | polymer | 101 | 11240.1 | 2 | UniProt (P43321) Pfam (PF01423) | Saccharomyces cerevisiae S288c | Sm-D3, snRNP core protein D3 |
| 11 | K, R (S, O) | Small nuclear ribonucleoprotein-associated protein B | polymer | 196 | 22427.0 | 2 | UniProt (P40018) Pfam (PF01423) | Saccharomyces cerevisiae S288c | snRNP-B, Sm protein B, Sm-B, SmB |
| 12 | L, S (T, P) | Small nuclear ribonucleoprotein Sm D1 | polymer | 146 | 16296.8 | 2 | UniProt (Q02260) Pfam (PF01423) | Saccharomyces cerevisiae S288c | Sm-D1, snRNP core protein D1 |
| 13 | M, T (U, Q) | Small nuclear ribonucleoprotein Sm D2 | polymer | 110 | 12876.1 | 2 | UniProt (Q06217) Pfam (PF01423) | Saccharomyces cerevisiae S288c | Sm-D2, snRNP core protein D2 |
| 14 | N, U (V, Y) | Small nuclear ribonucleoprotein E | polymer | 94 | 10385.1 | 2 | UniProt (Q12330) Pfam (PF01423) | Saccharomyces cerevisiae S288c | snRNP-E, Sm protein E, Sm-E, SmE |
| 15 | O, V (W, Z) | Small nuclear ribonucleoprotein F | polymer | 86 | 9669.9 | 2 | UniProt (P54999) Pfam (PF01423) | Saccharomyces cerevisiae S288c | snRNP-F, Sm protein F, Sm-F, SmF |
| 16 | P, W (X, a) | Small nuclear ribonucleoprotein G | polymer | 77 | 8490.8 | 2 | UniProt (P40204) Pfam (PF01423) | Saccharomyces cerevisiae S288c | snRNP-G, Sm protein G, Sm-G, SmG |
| 17 | X (b) | U6 snRNA-associated Sm-like protein LSm8 | polymer | 109 | 12403.4 | 1 | UniProt (P47093) Pfam (PF01423) | Saccharomyces cerevisiae S288c | |
| 18 | Y (c) | U6 snRNA-associated Sm-like protein LSm2 | polymer | 95 | 11177.9 | 1 | UniProt (P38203) Pfam (PF01423) | Saccharomyces cerevisiae S288c | Small nuclear ribonucleoprotein D homolog SNP3 |
| 19 | Z (d) | U6 snRNA-associated Sm-like protein LSm3 | polymer | 89 | 10039.3 | 1 | UniProt (P57743) Pfam (PF01423) | Saccharomyces cerevisiae S288c | SmX4 protein |
| 20 | AA (e) | U6 snRNA-associated Sm-like protein LSm6 | polymer | 86 | 9406.6 | 1 | UniProt (Q06406) Pfam (PF01423) | Saccharomyces cerevisiae S288c | |
| 21 | BA (f) | U6 snRNA-associated Sm-like protein LSm5 | polymer | 93 | 10433.0 | 1 | UniProt (P40089) Pfam (PF01423) | Saccharomyces cerevisiae S288c | |
| 22 | CA (g) | U6 snRNA-associated Sm-like protein LSm7 | polymer | 115 | 13027.0 | 1 | UniProt (P53905) Pfam (PF01423) | Saccharomyces cerevisiae S288c | |
| 23 | DA (h) | U6 snRNA-associated Sm-like protein LSm4 | polymer | 187 | 21298.1 | 1 | UniProt (P40070) Pfam (PF01423) | Saccharomyces cerevisiae S288c | |
| 24 | EA (C) | pre-mRNA | polymer | 20 | 6425.9 | 1 | synthetic construct | ||
| 25 | FA (D) | SNR6 snRNA | polymer | 112 | 35883.2 | 1 | GenBank (831416131) | Saccharomyces cerevisiae S288c | U6 snRNA |
| 26 | GA (E) | SNR14 snRNA | polymer | 160 | 51186.0 | 1 | GenBank (831416092) | Saccharomyces cerevisiae S288c | U4 snRNA |
| 27 | HA (F) | SNR7-L snRNA | polymer | 214 | 68643.3 | 1 | GenBank (831416109) | Saccharomyces cerevisiae S288c | U5 snRNA |
| 28 | IA (H) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 29 | JA (E) | N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) | non-polymer | 487.3 | 1 | Chemie (M7M) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 34 |
| Total formula weight | 1372822.2 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 1010.5 | |
| All* | Total formula weight | 1373832.7 |
