3JCM
Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Pre-mRNA-splicing factor 8 | polymer | 2413 | 279867.5 | 1 | UniProt (P33334) Pfam (PF08082) Pfam (PF08083) Pfam (PF10598) Pfam (PF10597) Pfam (PF10596) Pfam (PF12134) Pfam (PF08084) | Saccharomyces cerevisiae S288c | |
| 2 | B (B) | U4/U6 small nuclear ribonucleoprotein PRP4 | polymer | 465 | 52507.0 | 1 | UniProt (P20053) Pfam (PF00400) | Saccharomyces cerevisiae S288c | Pre-mRNA-processing protein 4 |
| 3 | C (I) | Pre-mRNA-processing factor 31 | polymer | 494 | 56382.5 | 1 | UniProt (P49704) Pfam (PF01798) Pfam (PF09785) | Saccharomyces cerevisiae S288c | |
| 4 | D (G) | Pre-mRNA-splicing factor 6 | polymer | 899 | 104370.1 | 1 | UniProt (P19735) Pfam (PF06424) | Saccharomyces cerevisiae S288c | |
| 5 | E (K) | U4/U6 small nuclear ribonucleoprotein PRP3 | polymer | 469 | 55974.3 | 1 | UniProt (Q03338) Pfam (PF08572) Pfam (PF06544) | Saccharomyces cerevisiae S288c | Pre-mRNA-splicing factor 3 |
| 6 | F (L) | Spliceosomal protein DIB1 | polymer | 143 | 16798.4 | 1 | UniProt (Q06819) Pfam (PF02966) | Saccharomyces cerevisiae S288c | |
| 7 | G (M) | 13 kDa ribonucleoprotein-associated protein | polymer | 126 | 13582.9 | 1 | UniProt (P39990) Pfam (PF01248) | Saccharomyces cerevisiae S288c | Small nuclear ribonucleoprotein-associated protein 1 |
| 8 | H (H) | Pre-mRNA-splicing factor SNU114 | polymer | 1008 | 114174.0 | 1 | UniProt (P36048) Pfam (PF16004) Pfam (PF00009) Pfam (PF03764) Pfam (PF00679) | Saccharomyces cerevisiae S288c | 114 kDa U5 small nuclear ribonucleoprotein component, Growth inhibitory protein 10 |
| 9 | I (N) | Pre-mRNA-splicing helicase BRR2 | polymer | 2163 | 246470.3 | 1 | UniProt (P32639) Pfam (PF21188) Pfam (PF18149) Pfam (PF00270) Pfam (PF00271) Pfam (PF02889) | Saccharomyces cerevisiae S288c | Protein Snu246 |
| 10 | J, Q (R, J) | Small nuclear ribonucleoprotein Sm D3 | polymer | 101 | 11240.1 | 2 | UniProt (P43321) Pfam (PF01423) | Saccharomyces cerevisiae S288c | Sm-D3, snRNP core protein D3 |
| 11 | K, R (S, O) | Small nuclear ribonucleoprotein-associated protein B | polymer | 196 | 22427.0 | 2 | UniProt (P40018) Pfam (PF01423) | Saccharomyces cerevisiae S288c | snRNP-B, Sm protein B, Sm-B, SmB |
| 12 | L, S (T, P) | Small nuclear ribonucleoprotein Sm D1 | polymer | 146 | 16296.8 | 2 | UniProt (Q02260) Pfam (PF01423) | Saccharomyces cerevisiae S288c | Sm-D1, snRNP core protein D1 |
| 13 | M, T (U, Q) | Small nuclear ribonucleoprotein Sm D2 | polymer | 110 | 12876.1 | 2 | UniProt (Q06217) Pfam (PF01423) | Saccharomyces cerevisiae S288c | Sm-D2, snRNP core protein D2 |
| 14 | N, U (V, Y) | Small nuclear ribonucleoprotein E | polymer | 94 | 10385.1 | 2 | UniProt (Q12330) Pfam (PF01423) | Saccharomyces cerevisiae S288c | snRNP-E, Sm protein E, Sm-E, SmE |
| 15 | O, V (W, Z) | Small nuclear ribonucleoprotein F | polymer | 86 | 9669.9 | 2 | UniProt (P54999) Pfam (PF01423) | Saccharomyces cerevisiae S288c | snRNP-F, Sm protein F, Sm-F, SmF |
| 16 | P, W (X, a) | Small nuclear ribonucleoprotein G | polymer | 77 | 8490.8 | 2 | UniProt (P40204) Pfam (PF01423) | Saccharomyces cerevisiae S288c | snRNP-G, Sm protein G, Sm-G, SmG |
| 17 | X (b) | U6 snRNA-associated Sm-like protein LSm8 | polymer | 109 | 12403.4 | 1 | UniProt (P47093) Pfam (PF01423) | Saccharomyces cerevisiae S288c | |
| 18 | Y (c) | U6 snRNA-associated Sm-like protein LSm2 | polymer | 95 | 11177.9 | 1 | UniProt (P38203) Pfam (PF01423) | Saccharomyces cerevisiae S288c | Small nuclear ribonucleoprotein D homolog SNP3 |
| 19 | Z (d) | U6 snRNA-associated Sm-like protein LSm3 | polymer | 89 | 10039.3 | 1 | UniProt (P57743) Pfam (PF01423) | Saccharomyces cerevisiae S288c | SmX4 protein |
| 20 | AA (e) | U6 snRNA-associated Sm-like protein LSm6 | polymer | 86 | 9406.6 | 1 | UniProt (Q06406) Pfam (PF01423) | Saccharomyces cerevisiae S288c | |
| 21 | BA (f) | U6 snRNA-associated Sm-like protein LSm5 | polymer | 93 | 10433.0 | 1 | UniProt (P40089) Pfam (PF01423) | Saccharomyces cerevisiae S288c | |
| 22 | CA (g) | U6 snRNA-associated Sm-like protein LSm7 | polymer | 115 | 13027.0 | 1 | UniProt (P53905) Pfam (PF01423) | Saccharomyces cerevisiae S288c | |
| 23 | DA (h) | U6 snRNA-associated Sm-like protein LSm4 | polymer | 187 | 21298.1 | 1 | UniProt (P40070) Pfam (PF01423) | Saccharomyces cerevisiae S288c | |
| 24 | EA (C) | pre-mRNA | polymer | 20 | 6425.9 | 1 | synthetic construct | ||
| 25 | FA (D) | SNR6 snRNA | polymer | 112 | 35883.2 | 1 | GenBank (831416131) | Saccharomyces cerevisiae S288c | U6 snRNA |
| 26 | GA (E) | SNR14 snRNA | polymer | 160 | 51186.0 | 1 | GenBank (831416092) | Saccharomyces cerevisiae S288c | U4 snRNA |
| 27 | HA (F) | SNR7-L snRNA | polymer | 214 | 68643.3 | 1 | GenBank (831416109) | Saccharomyces cerevisiae S288c | U5 snRNA |
| 28 | IA (H) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 29 | JA (E) | N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) | non-polymer | 487.3 | 1 | Chemie (M7M) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 34 |
| Total formula weight | 1372822.2 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 1010.5 | |
| All* | Total formula weight | 1373832.7 |
