3JAG
Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | uL2 | polymer | 244 | 26570.1 | 1 | UniProt (G1TT27) Pfam (PF00181) Pfam (PF03947) | Oryctolagus cuniculus (rabbit) | |
| 2 | B (B) | uL3 | polymer | 394 | 45119.8 | 1 | UniProt (G1TL06) Pfam (PF00297) | Oryctolagus cuniculus (rabbit) | |
| 3 | C (C) | uL4 | polymer | 362 | 41101.4 | 1 | UniProt (G1SVW5) Pfam (PF00573) Pfam (PF14374) | Oryctolagus cuniculus (rabbit) | |
| 4 | D (D) | uL18 | polymer | 292 | 34006.3 | 1 | UniProt (G1SYJ6) UniProt (by SIFTS) (P19949) | Oryctolagus cuniculus (rabbit) | |
| 5 | E (E) | eL6 | polymer | 248 | 28529.8 | 1 | UniProt (G1TG04) Pfam (PF01159) Pfam (PF03868) | Oryctolagus cuniculus (rabbit) | |
| 6 | F (F) | uL30 | polymer | 225 | 26662.8 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 7 | G (G) | eL8 | polymer | 241 | 27480.6 | 1 | UniProt (G1STW0) Pfam (PF01248) | Oryctolagus cuniculus (rabbit) | |
| 8 | H (H) | uL6 | polymer | 190 | 21627.2 | 1 | UniProt (G1TX33) Pfam (PF00347) UniProt (by SIFTS) (G1SWI6) | Oryctolagus cuniculus (rabbit) | |
| 9 | I (I) | uL16 | polymer | 213 | 24511.9 | 1 | UniProt (B7NZQ2) Pfam (PF00252) | Oryctolagus cuniculus (rabbit) | |
| 10 | J (J) | uL5 | polymer | 169 | 19270.3 | 1 | UniProt (G1TUB8) Pfam (PF00281) Pfam (PF00673) | Oryctolagus cuniculus (rabbit) | |
| 11 | K (L) | eL13 | polymer | 210 | 24216.5 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 12 | L (M) | eL14 | polymer | 138 | 16217.4 | 1 | UniProt (U3KNW6) Pfam (PF01929) UniProt (by SIFTS) (G1SZ12) | Oryctolagus cuniculus (rabbit) | |
| 13 | M (N) | eL15 | polymer | 203 | 24076.1 | 1 | UniProt (G1T0C1) Pfam (PF00827) | Oryctolagus cuniculus (rabbit) | |
| 14 | N (O) | uL13 | polymer | 199 | 23248.9 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 15 | O (P) | uL22 | polymer | 153 | 17758.7 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 16 | P (Q) | eL18 | polymer | 187 | 21457.4 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 17 | Q (R) | eL19 | polymer | 180 | 21613.0 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 18 | R (S) | eL20 | polymer | 175 | 20696.4 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 19 | S (T) | eL21 | polymer | 159 | 18478.8 | 1 | UniProt (G1SHQ2) Pfam (PF01157) | Oryctolagus cuniculus (rabbit) | |
| 20 | T (U) | eL22 | polymer | 99 | 11481.2 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 21 | U (V) | uL14 | polymer | 131 | 13972.4 | 1 | UniProt (G1T6D1) Pfam (PF00238) | Oryctolagus cuniculus (rabbit) | |
| 22 | V (W) | eL24 | polymer | 63 | 7512.8 | 1 | UniProt (G1TLD5) | Oryctolagus cuniculus (rabbit) | |
| 23 | W (X) | uL23 | polymer | 119 | 13856.4 | 1 | UniProt (G1SE76) Pfam (PF03939) Pfam (PF00276) | Oryctolagus cuniculus (rabbit) | |
| 24 | X (Y) | uL24 | polymer | 134 | 15891.8 | 1 | UniProt (G1SQH0) Pfam (PF16906) Pfam (PF00467) | Oryctolagus cuniculus (rabbit) | |
| 25 | Y (Z) | eL27 | polymer | 135 | 15704.6 | 1 | UniProt (G1TXF6) Pfam (PF00467) Pfam (PF01777) | Oryctolagus cuniculus (rabbit) | |
| 26 | Z (a) | uL15 | polymer | 147 | 16489.4 | 1 | UniProt (G1SNY0) Pfam (PF00828) | Oryctolagus cuniculus (rabbit) | |
| 27 | AA (b) | eL29 | polymer | 75 | 8706.3 | 1 | UniProt (G1SGR6) Pfam (PF01779) | Oryctolagus cuniculus (rabbit) | |
| 28 | BA (c) | eL30 | polymer | 94 | 10496.4 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 29 | CA (d) | eL31 | polymer | 107 | 12635.7 | 1 | UniProt (G1SHG0) Pfam (PF01198) | Oryctolagus cuniculus (rabbit) | |
| 30 | DA (e) | eL32 | polymer | 128 | 15022.0 | 1 | UniProt (G1TUN8) Pfam (PF01655) | Oryctolagus cuniculus (rabbit) | |
| 31 | EA (f) | eL33 | polymer | 109 | 12449.6 | 1 | UniProt (G1SF08) Pfam (PF01247) | Oryctolagus cuniculus (rabbit) | |
| 32 | FA (g) | eL34 | polymer | 114 | 12994.6 | 1 | UniProt (G1U945) Pfam (PF01199) UniProt (by SIFTS) (G1TXG5) | Oryctolagus cuniculus (rabbit) | |
| 33 | GA (h) | uL29 | polymer | 122 | 14435.4 | 1 | UniProt (G1SIT5) Pfam (PF00831) | Oryctolagus cuniculus (rabbit) | |
| 34 | HA (i) | eL36 | polymer | 102 | 11888.4 | 1 | UniProt (G1TTQ5) Pfam (PF01158) | Oryctolagus cuniculus (rabbit) | |
| 35 | IA (j) | eL37 | polymer | 86 | 10090.9 | 1 | UniProt (U3KPD5) Pfam (PF01907) | Oryctolagus cuniculus (rabbit) | |
| 36 | JA (k) | eL38 | polymer | 69 | 8107.8 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 37 | KA (l) | eL39 | polymer | 50 | 6295.6 | 1 | UniProt (G1SYU7) Pfam (PF00832) | Oryctolagus cuniculus (rabbit) | |
| 38 | LA (m) | eL40 | polymer | 52 | 6199.6 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 39 | MA (n) | eL41 | polymer | 23 | 3213.1 | 1 | UniProt (A0A087WNH4) Pfam (PF05162) | Oryctolagus cuniculus (rabbit) | |
| 40 | NA (o) | eL42 | polymer | 104 | 12198.6 | 1 | UniProt (G1T040) Pfam (PF00935) | Oryctolagus cuniculus (rabbit) | |
| 41 | OA (p) | eL43 | polymer | 91 | 10168.2 | 1 | UniProt (G1SY53) Pfam (PF01780) | Oryctolagus cuniculus (rabbit) | |
| 42 | PA (r) | eL28 | polymer | 125 | 14319.8 | 1 | UniProt (G1U7L1) Pfam (PF01778) | Oryctolagus cuniculus (rabbit) | |
| 43 | QA (s) | uL10 | polymer | 198 | 21747.3 | 1 | UniProt (G1U6N2) | Oryctolagus cuniculus (rabbit) | |
| 44 | RA (t) | uL11 | polymer | 163 | 17689.5 | 1 | UniProt (G1SMR7) Pfam (PF03946) Pfam (PF00298) | Oryctolagus cuniculus (rabbit) | |
| 45 | SA (1) | peptide | polymer | 15 | 1788.0 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 46 | TA (2) | tRNA(Val) | polymer | 76 | 24436.6 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 47 | UA (3) | tRNA(Lys) | polymer | 75 | 24102.3 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 48 | VA (5) | 28S ribosomal RNA | polymer | 3662 | 1186579.5 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 49 | WA (7) | 5S ribosomal RNA | polymer | 120 | 38691.9 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 50 | XA (8) | 5.8S ribosomal RNA | polymer | 156 | 50143.6 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 51 | YA (9) | 18S ribosomal RNA | polymer | 1719 | 554751.3 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 52 | ZA (AA) | uS2 | polymer | 208 | 23360.8 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 53 | AB (BB) | eS1 | polymer | 213 | 24759.1 | 1 | UniProt (G1SS70) Pfam (PF01015) | Oryctolagus cuniculus (rabbit) | |
| 54 | BB (CC) | uS5 | polymer | 218 | 24080.4 | 1 | UniProt (G1TUT9) | Oryctolagus cuniculus (rabbit) | |
| 55 | CB (DD) | uS3 | polymer | 227 | 25158.5 | 1 | UniProt (G1TNM3) Pfam (PF07650) Pfam (PF00189) | Oryctolagus cuniculus (rabbit) | |
| 56 | DB (EE) | eS4 | polymer | 262 | 29497.7 | 1 | UniProt (G1TK17) Pfam (PF08071) Pfam (PF00900) Pfam (PF00467) Pfam (PF16121) | Oryctolagus cuniculus (rabbit) | |
| 57 | EB (FF) | uS7 | polymer | 191 | 21525.9 | 1 | UniProt (G1TFM5) Pfam (PF00177) | Oryctolagus cuniculus (rabbit) | |
| 58 | FB (GG) | eS6 | polymer | 237 | 27471.5 | 1 | UniProt (G1TM55) Pfam (PF01092) | Oryctolagus cuniculus (rabbit) | |
| 59 | GB (HH) | eS7 | polymer | 189 | 21603.2 | 1 | UniProt (G1SVB0) | Oryctolagus cuniculus (rabbit) | |
| 60 | HB (II) | eS8 | polymer | 206 | 24003.0 | 1 | UniProt (G1TJW1) Pfam (PF01201) | Oryctolagus cuniculus (rabbit) | |
| 61 | IB (JJ) | uS4 | polymer | 185 | 21649.6 | 1 | UniProt (B7NZS8) Pfam (PF00163) Pfam (PF01479) | Oryctolagus cuniculus (rabbit) | |
| 62 | JB (KK) | eS10 | polymer | 98 | 11774.0 | 1 | UniProt (G1TPV3) Pfam (PF03501) | Oryctolagus cuniculus (rabbit) | |
| 63 | KB (LL) | uS17 | polymer | 152 | 17657.8 | 1 | UniProt (G1TRM4) Pfam (PF16205) Pfam (PF00366) | Oryctolagus cuniculus (rabbit) | |
| 64 | LB (MM) | eS12 | polymer | 124 | 13766.1 | 1 | UniProt (G1SFR8) Pfam (PF01248) | Oryctolagus cuniculus (rabbit) | |
| 65 | MB (NN) | uS15 | polymer | 150 | 17128.2 | 1 | UniProt (G1SP51) Pfam (PF08069) Pfam (PF00312) | Oryctolagus cuniculus (rabbit) | |
| 66 | NB (OO) | uS11 | polymer | 136 | 14544.7 | 1 | UniProt (G1T1F0) Pfam (PF00411) | Oryctolagus cuniculus (rabbit) | |
| 67 | OB (PP) | uS19 | polymer | 127 | 15151.9 | 1 | UniProt (G1U0Q2) Pfam (PF00203) | Oryctolagus cuniculus (rabbit) | |
| 68 | PB (QQ) | uS9 | polymer | 141 | 15975.8 | 1 | UniProt (G1SGX4) Pfam (PF00380) | Oryctolagus cuniculus (rabbit) | |
| 69 | QB (RR) | eS17 | polymer | 129 | 14969.4 | 1 | UniProt (G1TU13) Pfam (PF00833) | Oryctolagus cuniculus (rabbit) | |
| 70 | RB (SS) | uS13 | polymer | 137 | 16170.8 | 1 | UniProt (G1SZI5) Pfam (PF00416) UniProt (by SIFTS) (G1TPG3) | Oryctolagus cuniculus (rabbit) | |
| 71 | SB (TT) | eS19 | polymer | 141 | 15683.1 | 1 | UniProt (G1TN62) Pfam (PF01090) | Oryctolagus cuniculus (rabbit) | |
| 72 | TB (UU) | uS10 | polymer | 104 | 11765.9 | 1 | UniProt (G1SIZ2) Pfam (PF00338) | Oryctolagus cuniculus (rabbit) | |
| 73 | UB (VV) | eS21 | polymer | 83 | 9115.4 | 1 | UniProt (G1TM82) Pfam (PF01249) | Oryctolagus cuniculus (rabbit) | |
| 74 | VB (WW) | uS8 | polymer | 129 | 14734.4 | 1 | UniProt (G1TG89) Pfam (PF00410) | Oryctolagus cuniculus (rabbit) | |
| 75 | WB (XX) | uS12 | polymer | 141 | 15626.4 | 1 | UniProt (G1SZ47) Pfam (PF00164) | Oryctolagus cuniculus (rabbit) | |
| 76 | XB (YY) | eS24 | polymer | 126 | 14604.3 | 1 | UniProt (G1T3D8) Pfam (PF01282) | Oryctolagus cuniculus (rabbit) | |
| 77 | YB (ZZ) | eS25 | polymer | 75 | 8526.1 | 1 | UniProt (G1TDB3) Pfam (PF03297) | Oryctolagus cuniculus (rabbit) | |
| 78 | ZB (aa) | eS26 | polymer | 98 | 11184.2 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 79 | AC (bb) | eS27 | polymer | 83 | 9349.0 | 1 | UniProt (G1TZ76) Pfam (PF01667) | Oryctolagus cuniculus (rabbit) | |
| 80 | BC (cc) | eS28 | polymer | 61 | 6819.8 | 1 | UniProt (G1TIB4) Pfam (PF01200) | Oryctolagus cuniculus (rabbit) | |
| 81 | CC (dd) | uS14 | polymer | 53 | 6364.4 | 1 | UniProt (G1U7M4) Pfam (PF00253) | Oryctolagus cuniculus (rabbit) | |
| 82 | DC (ee) | eS30 | polymer | 57 | 6512.7 | 1 | UniProt (G1T8A2) Pfam (PF04758) | Oryctolagus cuniculus (rabbit) | |
| 83 | EC (ff) | eS31 | polymer | 69 | 8100.5 | 1 | UniProt (G1SK22) Pfam (PF00240) Pfam (PF01599) | Oryctolagus cuniculus (rabbit) | |
| 84 | FC (gg) | RACK1 | polymer | 313 | 34669.1 | 1 | UniProt (G1SJB4) Pfam (PF00400) | Oryctolagus cuniculus (rabbit) | |
| 85 | GC (hh) | mRNA | polymer | 12 | 3821.3 | 1 | Oryctolagus cuniculus (rabbit) | ||
| 86 | HC (ii) | eRF1 | polymer | 416 | 46650.3 | 1 | UniProt (P62495) Pfam (PF03463) Pfam (PF03464) Pfam (PF03465) | Homo sapiens (human) | |
| 87 | IC (jj) | ABCE1 | polymer | 594 | 66857.7 | 1 | UniProt (G1SG72) Pfam (PF04068) Pfam (PF00037) Pfam (PF00005) | Oryctolagus cuniculus (rabbit) | |
| 88 | AD, AE, AF, AG, AH... (5, 9, hh, C, I...) | MAGNESIUM ION | non-polymer | 24.3 | 195 | Chemie (MG) | |||
| 89 | AK, BK, CK, OC, QC... (aa, dd, ff, g, j...) | ZINC ION | non-polymer | 65.4 | 8 | Chemie (ZN) | |||
| 90 | EK, FK (jj) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 2 | Chemie (SF4) | |||
| 91 | GK, HK (jj) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 2 | Chemie (ADP) |
Sequence modifications
C: 2 - 360 (UniProt: G1SVW5)
G: 26 - 266 (UniProt: G1STW0)
CC: 59 - 276 (UniProt: G1TUT9)
EE: 2 - 263 (UniProt: G1TK17)
II: 2 - 207 (UniProt: G1TJW1)
TT: 4 - 144 (UniProt: G1TN62)
VV: 1 - 83 (UniProt: G1TM82)
cc: 8 - 68 (UniProt: G1TIB4)
ii: 6 - 421 (UniProt: P62495)
| PDB | External Database | Details |
|---|---|---|
| Lys 361 | - | expression tag |
| Ser 362 | - | expression tag |
| Asp 363 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 191 | Cys 167 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Arg 194 | His 174 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Gly 25 | Ser 25 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Arg 47 | Gly 47 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Gly 119 | Trp 119 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Asn 3 | Ser 3 | conflict |
| Asp 4 | Asn 4 | conflict |
| Phe 50 | Ser 50 | conflict |
| Ala 75 | Ser 75 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ile 18 | Leu 18 | conflict |
| Lys 20 | Arg 20 | conflict |
| His 40 | Arg 40 | conflict |
| Thr 42 | Ile 42 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 183 | Gly 183 | engineered mutation |
| Ala 184 | Gly 184 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 87 |
| Total formula weight | 3337337.1 | |
| Non-Polymers* | Number of molecules | 207 |
| Total formula weight | 6820.4 | |
| All* | Total formula weight | 3344157.5 |
