3JAG
Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon
This is a non-PDB format compatible entry.
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK III |
Method | After 30 second wait time, blot for 3 seconds before plunging |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | FEI FALCON II (4k x 4k) |
Number of Images | 2672 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.65 |
Software | CTFFIND,Coot,UCSF Chimera,RELION,EPU |
Symmetry | C1 |