3J99

Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State IIIb)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Vesicle-fusing ATPase	polymer	747	82907.4	6	UniProt (P18708) Pfam (PF02359) Pfam (PF02933) Pfam (PF00004) Pfam (PF17862) Pfam (PF21964)	Cricetulus griseus (Chinese hamster)	N-ethylmaleimide-sensitive fusion protein, NEM-sensitive fusion protein, Vesicular-fusion protein NSF, N-ethylmaleimide sensitive factor
2	G, H, I, J (H, I, J, G)	Alpha-soluble NSF attachment protein	polymer	297	33377.8	4	UniProt (P54921)	Rattus norvegicus (rat)	SNAP-alpha, N-ethylmaleimide-sensitive factor attachment protein alpha, alpha-SNAP
3	K (K)	Vesicle-associated membrane protein 2	polymer	63	7231.1	1	UniProt (P63045) Pfam (PF00957)	Rattus norvegicus (rat)	VAMP-2, Synaptobrevin-2
4	L (L)	Syntaxin-1A	polymer	67	7838.0	1	UniProt (P32851) Pfam (PF05739)	Rattus norvegicus (rat)	Neuron-specific antigen HPC-1, Synaptotagmin-associated 35 kDa protein, P35A
5	M (M)	Synaptosomal-associated protein 25	polymer	188	21256.9	1	Pfam (PF00835) UniProt (by SIFTS) (P60881)	Rattus norvegicus (rat)	SNAP-25, Super protein, SUP, Synaptosomal-associated 25 kDa protein

Sequence modifications

A, B, C, D, E, F: 1 - 744 (UniProt: P18708)

PDB	External Database	Details
Gly -2	-	expression tag
Ala -1	-	expression tag
His 0	-	expression tag

H, I, J, G: 1 - 295 (UniProt: P54921)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

K: 28 - 89 (UniProt: P63045)

PDB	External Database	Details
Gly 27	-	expression tag

L: 191 - 256 (UniProt: P32851)

PDB	External Database	Details
Met 190	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	13
	Total formula weight	667281.6
All*	Total formula weight	667281.6

*Water molecules are not included.