3J99

Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State IIIb)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000149	molecular_function	SNARE binding
A	0000166	molecular_function	nucleotide binding
A	0001921	biological_process	positive regulation of receptor recycling
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005794	cellular_component	Golgi apparatus
A	0005795	cellular_component	Golgi stack
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006813	biological_process	potassium ion transport
A	0006886	biological_process	intracellular protein transport
A	0006891	biological_process	intra-Golgi vesicle-mediated transport
A	0015031	biological_process	protein transport
A	0016787	molecular_function	hydrolase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0017075	molecular_function	syntaxin-1 binding
A	0019901	molecular_function	protein kinase binding
A	0019905	molecular_function	syntaxin binding
A	0030165	molecular_function	PDZ domain binding
A	0030496	cellular_component	midbody
A	0035255	molecular_function	ionotropic glutamate receptor binding
A	0035494	biological_process	SNARE complex disassembly
A	0042802	molecular_function	identical protein binding
A	0043001	biological_process	Golgi to plasma membrane protein transport
A	0044877	molecular_function	protein-containing complex binding
A	0045732	biological_process	positive regulation of protein catabolic process
A	0046872	molecular_function	metal ion binding
A	0140545	molecular_function	ATP-dependent protein disaggregase activity
B	0000149	molecular_function	SNARE binding
B	0000166	molecular_function	nucleotide binding
B	0001921	biological_process	positive regulation of receptor recycling
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005794	cellular_component	Golgi apparatus
B	0005795	cellular_component	Golgi stack
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006813	biological_process	potassium ion transport
B	0006886	biological_process	intracellular protein transport
B	0006891	biological_process	intra-Golgi vesicle-mediated transport
B	0015031	biological_process	protein transport
B	0016787	molecular_function	hydrolase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0017075	molecular_function	syntaxin-1 binding
B	0019901	molecular_function	protein kinase binding
B	0019905	molecular_function	syntaxin binding
B	0030165	molecular_function	PDZ domain binding
B	0030496	cellular_component	midbody
B	0035255	molecular_function	ionotropic glutamate receptor binding
B	0035494	biological_process	SNARE complex disassembly
B	0042802	molecular_function	identical protein binding
B	0043001	biological_process	Golgi to plasma membrane protein transport
B	0044877	molecular_function	protein-containing complex binding
B	0045732	biological_process	positive regulation of protein catabolic process
B	0046872	molecular_function	metal ion binding
B	0140545	molecular_function	ATP-dependent protein disaggregase activity
C	0000149	molecular_function	SNARE binding
C	0000166	molecular_function	nucleotide binding
C	0001921	biological_process	positive regulation of receptor recycling
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005794	cellular_component	Golgi apparatus
C	0005795	cellular_component	Golgi stack
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006813	biological_process	potassium ion transport
C	0006886	biological_process	intracellular protein transport
C	0006891	biological_process	intra-Golgi vesicle-mediated transport
C	0015031	biological_process	protein transport
C	0016787	molecular_function	hydrolase activity
C	0016887	molecular_function	ATP hydrolysis activity
C	0017075	molecular_function	syntaxin-1 binding
C	0019901	molecular_function	protein kinase binding
C	0019905	molecular_function	syntaxin binding
C	0030165	molecular_function	PDZ domain binding
C	0030496	cellular_component	midbody
C	0035255	molecular_function	ionotropic glutamate receptor binding
C	0035494	biological_process	SNARE complex disassembly
C	0042802	molecular_function	identical protein binding
C	0043001	biological_process	Golgi to plasma membrane protein transport
C	0044877	molecular_function	protein-containing complex binding
C	0045732	biological_process	positive regulation of protein catabolic process
C	0046872	molecular_function	metal ion binding
C	0140545	molecular_function	ATP-dependent protein disaggregase activity
D	0000149	molecular_function	SNARE binding
D	0000166	molecular_function	nucleotide binding
D	0001921	biological_process	positive regulation of receptor recycling
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005794	cellular_component	Golgi apparatus
D	0005795	cellular_component	Golgi stack
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0006813	biological_process	potassium ion transport
D	0006886	biological_process	intracellular protein transport
D	0006891	biological_process	intra-Golgi vesicle-mediated transport
D	0015031	biological_process	protein transport
D	0016787	molecular_function	hydrolase activity
D	0016887	molecular_function	ATP hydrolysis activity
D	0017075	molecular_function	syntaxin-1 binding
D	0019901	molecular_function	protein kinase binding
D	0019905	molecular_function	syntaxin binding
D	0030165	molecular_function	PDZ domain binding
D	0030496	cellular_component	midbody
D	0035255	molecular_function	ionotropic glutamate receptor binding
D	0035494	biological_process	SNARE complex disassembly
D	0042802	molecular_function	identical protein binding
D	0043001	biological_process	Golgi to plasma membrane protein transport
D	0044877	molecular_function	protein-containing complex binding
D	0045732	biological_process	positive regulation of protein catabolic process
D	0046872	molecular_function	metal ion binding
D	0140545	molecular_function	ATP-dependent protein disaggregase activity
E	0000149	molecular_function	SNARE binding
E	0000166	molecular_function	nucleotide binding
E	0001921	biological_process	positive regulation of receptor recycling
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005794	cellular_component	Golgi apparatus
E	0005795	cellular_component	Golgi stack
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0006813	biological_process	potassium ion transport
E	0006886	biological_process	intracellular protein transport
E	0006891	biological_process	intra-Golgi vesicle-mediated transport
E	0015031	biological_process	protein transport
E	0016787	molecular_function	hydrolase activity
E	0016887	molecular_function	ATP hydrolysis activity
E	0017075	molecular_function	syntaxin-1 binding
E	0019901	molecular_function	protein kinase binding
E	0019905	molecular_function	syntaxin binding
E	0030165	molecular_function	PDZ domain binding
E	0030496	cellular_component	midbody
E	0035255	molecular_function	ionotropic glutamate receptor binding
E	0035494	biological_process	SNARE complex disassembly
E	0042802	molecular_function	identical protein binding
E	0043001	biological_process	Golgi to plasma membrane protein transport
E	0044877	molecular_function	protein-containing complex binding
E	0045732	biological_process	positive regulation of protein catabolic process
E	0046872	molecular_function	metal ion binding
E	0140545	molecular_function	ATP-dependent protein disaggregase activity
F	0000149	molecular_function	SNARE binding
F	0000166	molecular_function	nucleotide binding
F	0001921	biological_process	positive regulation of receptor recycling
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005794	cellular_component	Golgi apparatus
F	0005795	cellular_component	Golgi stack
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0006813	biological_process	potassium ion transport
F	0006886	biological_process	intracellular protein transport
F	0006891	biological_process	intra-Golgi vesicle-mediated transport
F	0015031	biological_process	protein transport
F	0016787	molecular_function	hydrolase activity
F	0016887	molecular_function	ATP hydrolysis activity
F	0017075	molecular_function	syntaxin-1 binding
F	0019901	molecular_function	protein kinase binding
F	0019905	molecular_function	syntaxin binding
F	0030165	molecular_function	PDZ domain binding
F	0030496	cellular_component	midbody
F	0035255	molecular_function	ionotropic glutamate receptor binding
F	0035494	biological_process	SNARE complex disassembly
F	0042802	molecular_function	identical protein binding
F	0043001	biological_process	Golgi to plasma membrane protein transport
F	0044877	molecular_function	protein-containing complex binding
F	0045732	biological_process	positive regulation of protein catabolic process
F	0046872	molecular_function	metal ion binding
F	0140545	molecular_function	ATP-dependent protein disaggregase activity
G	0000149	molecular_function	SNARE binding
G	0005483	molecular_function	soluble NSF attachment protein activity
G	0005515	molecular_function	protein binding
G	0005774	cellular_component	vacuolar membrane
G	0005886	cellular_component	plasma membrane
G	0006886	biological_process	intracellular protein transport
G	0007420	biological_process	brain development
G	0010807	biological_process	regulation of synaptic vesicle priming
G	0015031	biological_process	protein transport
G	0016020	cellular_component	membrane
G	0016082	biological_process	synaptic vesicle priming
G	0016192	biological_process	vesicle-mediated transport
G	0019905	molecular_function	syntaxin binding
G	0030182	biological_process	neuron differentiation
G	0031594	cellular_component	neuromuscular junction
G	0032781	biological_process	positive regulation of ATP-dependent activity
G	0032984	biological_process	protein-containing complex disassembly
G	0035249	biological_process	synaptic transmission, glutamatergic
G	0035494	biological_process	SNARE complex disassembly
G	0043195	cellular_component	terminal bouton
G	0044877	molecular_function	protein-containing complex binding
G	0045176	biological_process	apical protein localization
G	0048787	cellular_component	presynaptic active zone membrane
G	0061025	biological_process	membrane fusion
G	0070044	cellular_component	synaptobrevin 2-SNAP-25-syntaxin-1a complex
G	0098793	cellular_component	presynapse
G	0098794	cellular_component	postsynapse
G	0098978	cellular_component	glutamatergic synapse
H	0000149	molecular_function	SNARE binding
H	0005483	molecular_function	soluble NSF attachment protein activity
H	0005515	molecular_function	protein binding
H	0005774	cellular_component	vacuolar membrane
H	0005886	cellular_component	plasma membrane
H	0006886	biological_process	intracellular protein transport
H	0007420	biological_process	brain development
H	0010807	biological_process	regulation of synaptic vesicle priming
H	0015031	biological_process	protein transport
H	0016020	cellular_component	membrane
H	0016082	biological_process	synaptic vesicle priming
H	0016192	biological_process	vesicle-mediated transport
H	0019905	molecular_function	syntaxin binding
H	0030182	biological_process	neuron differentiation
H	0031594	cellular_component	neuromuscular junction
H	0032781	biological_process	positive regulation of ATP-dependent activity
H	0032984	biological_process	protein-containing complex disassembly
H	0035249	biological_process	synaptic transmission, glutamatergic
H	0035494	biological_process	SNARE complex disassembly
H	0043195	cellular_component	terminal bouton
H	0044877	molecular_function	protein-containing complex binding
H	0045176	biological_process	apical protein localization
H	0048787	cellular_component	presynaptic active zone membrane
H	0061025	biological_process	membrane fusion
H	0070044	cellular_component	synaptobrevin 2-SNAP-25-syntaxin-1a complex
H	0098793	cellular_component	presynapse
H	0098794	cellular_component	postsynapse
H	0098978	cellular_component	glutamatergic synapse
I	0000149	molecular_function	SNARE binding
I	0005483	molecular_function	soluble NSF attachment protein activity
I	0005515	molecular_function	protein binding
I	0005774	cellular_component	vacuolar membrane
I	0005886	cellular_component	plasma membrane
I	0006886	biological_process	intracellular protein transport
I	0007420	biological_process	brain development
I	0010807	biological_process	regulation of synaptic vesicle priming
I	0015031	biological_process	protein transport
I	0016020	cellular_component	membrane
I	0016082	biological_process	synaptic vesicle priming
I	0016192	biological_process	vesicle-mediated transport
I	0019905	molecular_function	syntaxin binding
I	0030182	biological_process	neuron differentiation
I	0031594	cellular_component	neuromuscular junction
I	0032781	biological_process	positive regulation of ATP-dependent activity
I	0032984	biological_process	protein-containing complex disassembly
I	0035249	biological_process	synaptic transmission, glutamatergic
I	0035494	biological_process	SNARE complex disassembly
I	0043195	cellular_component	terminal bouton
I	0044877	molecular_function	protein-containing complex binding
I	0045176	biological_process	apical protein localization
I	0048787	cellular_component	presynaptic active zone membrane
I	0061025	biological_process	membrane fusion
I	0070044	cellular_component	synaptobrevin 2-SNAP-25-syntaxin-1a complex
I	0098793	cellular_component	presynapse
I	0098794	cellular_component	postsynapse
I	0098978	cellular_component	glutamatergic synapse
J	0000149	molecular_function	SNARE binding
J	0005483	molecular_function	soluble NSF attachment protein activity
J	0005515	molecular_function	protein binding
J	0005774	cellular_component	vacuolar membrane
J	0005886	cellular_component	plasma membrane
J	0006886	biological_process	intracellular protein transport
J	0007420	biological_process	brain development
J	0010807	biological_process	regulation of synaptic vesicle priming
J	0015031	biological_process	protein transport
J	0016020	cellular_component	membrane
J	0016082	biological_process	synaptic vesicle priming
J	0016192	biological_process	vesicle-mediated transport
J	0019905	molecular_function	syntaxin binding
J	0030182	biological_process	neuron differentiation
J	0031594	cellular_component	neuromuscular junction
J	0032781	biological_process	positive regulation of ATP-dependent activity
J	0032984	biological_process	protein-containing complex disassembly
J	0035249	biological_process	synaptic transmission, glutamatergic
J	0035494	biological_process	SNARE complex disassembly
J	0043195	cellular_component	terminal bouton
J	0044877	molecular_function	protein-containing complex binding
J	0045176	biological_process	apical protein localization
J	0048787	cellular_component	presynaptic active zone membrane
J	0061025	biological_process	membrane fusion
J	0070044	cellular_component	synaptobrevin 2-SNAP-25-syntaxin-1a complex
J	0098793	cellular_component	presynapse
J	0098794	cellular_component	postsynapse
J	0098978	cellular_component	glutamatergic synapse
K	0016020	cellular_component	membrane
K	0016192	biological_process	vesicle-mediated transport
L	0005484	molecular_function	SNAP receptor activity
L	0006886	biological_process	intracellular protein transport
L	0016020	cellular_component	membrane
L	0016192	biological_process	vesicle-mediated transport
M	0000149	molecular_function	SNARE binding
M	0001917	cellular_component	photoreceptor inner segment
M	0005249	molecular_function	voltage-gated potassium channel activity
M	0005484	molecular_function	SNAP receptor activity
M	0005515	molecular_function	protein binding
M	0005737	cellular_component	cytoplasm
M	0005768	cellular_component	endosome
M	0005802	cellular_component	trans-Golgi network
M	0005856	cellular_component	cytoskeleton
M	0005886	cellular_component	plasma membrane
M	0005938	cellular_component	cell cortex
M	0006887	biological_process	exocytosis
M	0007269	biological_process	neurotransmitter secretion
M	0007409	biological_process	axonogenesis
M	0007616	biological_process	long-term memory
M	0007626	biological_process	locomotory behavior
M	0008021	cellular_component	synaptic vesicle
M	0008076	cellular_component	voltage-gated potassium channel complex
M	0008289	molecular_function	lipid binding
M	0008306	biological_process	associative learning
M	0010975	biological_process	regulation of neuron projection development
M	0015629	cellular_component	actin cytoskeleton
M	0016020	cellular_component	membrane
M	0016079	biological_process	synaptic vesicle exocytosis
M	0016082	biological_process	synaptic vesicle priming
M	0016197	biological_process	endosomal transport
M	0017022	molecular_function	myosin binding
M	0017075	molecular_function	syntaxin-1 binding
M	0019904	molecular_function	protein domain specific binding
M	0030027	cellular_component	lamellipodium
M	0030175	cellular_component	filopodium
M	0030182	biological_process	neuron differentiation
M	0030424	cellular_component	axon
M	0030426	cellular_component	growth cone
M	0031083	cellular_component	BLOC-1 complex
M	0031201	cellular_component	SNARE complex
M	0031629	biological_process	synaptic vesicle fusion to presynaptic active zone membrane
M	0031982	cellular_component	vesicle
M	0032024	biological_process	positive regulation of insulin secretion
M	0035493	biological_process	SNARE complex assembly
M	0036477	cellular_component	somatodendritic compartment
M	0042734	cellular_component	presynaptic membrane
M	0043005	cellular_component	neuron projection
M	0043025	cellular_component	neuronal cell body
M	0043195	cellular_component	terminal bouton
M	0044295	cellular_component	axonal growth cone
M	0044325	molecular_function	transmembrane transporter binding
M	0045202	cellular_component	synapse
M	0046887	biological_process	positive regulation of hormone secretion
M	0048306	molecular_function	calcium-dependent protein binding
M	0048471	cellular_component	perinuclear region of cytoplasm
M	0048787	cellular_component	presynaptic active zone membrane
M	0048791	biological_process	calcium ion-regulated exocytosis of neurotransmitter
M	0051963	biological_process	regulation of synapse assembly
M	0060291	biological_process	long-term synaptic potentiation
M	0070032	cellular_component	synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex
M	0070033	cellular_component	synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex
M	0070044	cellular_component	synaptobrevin 2-SNAP-25-syntaxin-1a complex
M	0070201	biological_process	regulation of establishment of protein localization
M	0071805	biological_process	potassium ion transmembrane transport
M	0097470	cellular_component	ribbon synapse
M	0098793	cellular_component	presynapse
M	0098794	cellular_component	postsynapse
M	0098888	cellular_component	extrinsic component of presynaptic membrane
M	0098967	biological_process	exocytic insertion of neurotransmitter receptor to postsynaptic membrane
M	0098978	cellular_component	glutamatergic synapse
M	0099525	biological_process	presynaptic dense core vesicle exocytosis
M	0099590	biological_process	neurotransmitter receptor internalization

Functional Information from PROSITE/UniProt

site_id	PS00914
Number of Residues	40
Details	SYNTAXIN Syntaxin / epimorphin family signature. RhseIikLEnsIrELhdMFmdMamlVesQGemIDrIEyn.V

Chain	Residue	Details
L	ARG198-VAL237

---

site_id	PS00417
Number of Residues	20
Details	SYNAPTOBREVIN Synaptobrevin signature. NVDKVlERdqKLSeLdDRAD

Chain	Residue	Details
K	ASN49-ASP68

---

site_id	PS00674
Number of Residues	19
Details	AAA AAA-protein family signature. IlVIgMTNrpdlIDeALl.R

Chain	Residue	Details
A	ILE367-ARG385

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	SITE: (Microbial infection) Cleavage; by C.botulinum neurotoxin type E (BoNT/E) => ECO:0000269|PubMed:8243676, ECO:0000269|PubMed:8294407, ECO:0000305|PubMed:8103915

Chain	Residue	Details
M	ARG180
E	PRO545
F	ASN505
F	PRO545
I	MET1
J	MET1
G	MET1
C	ASN505
C	PRO545
D	ASN505
D	PRO545
E	ASN505

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	SITE: (Microbial infection) Cleavage; by C.botulinum neurotoxin type A (BoNT/A, botA) => ECO:0000269|PubMed:8243676, ECO:0000269|PubMed:8294407, ECO:0000305|PubMed:8103915

Chain	Residue	Details
M	GLN197
I	SER26
L	LYS253
L	LYS256
E	THR550
F	THR550

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphothreonine; by PKC and PKA => ECO:0000269|PubMed:12459461

Chain	Residue	Details
M	THR138
H	SER195
I	SER29
I	SER195
J	SER29
J	SER195
G	SER29
G	SER195

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P60879

Chain	Residue	Details
M	SER154
B	SER207
C	SER207
D	SER207
E	SER207
F	SER207

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine; by PKC => ECO:0000269|PubMed:12459461

Chain	Residue	Details
M	SER187
B	TYR259
C	TYR259
D	TYR259
E	TYR259
F	TYR259

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	LIPID: S-palmitoyl cysteine => ECO:0000250|UniProtKB:P60879

Chain	Residue	Details
M	CYS85
M	CYS88
M	CYS90
M	CYS92
E	SER569
F	SER569

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 642

Chain	Residue	Details
A	LYS549	electrostatic stabiliser
A	THR550	electrostatic stabiliser
A	ASP603	electrostatic stabiliser
A	ASP604	proton acceptor
A	LYS631	electrostatic stabiliser
A	LYS708	electrostatic stabiliser

---

site_id	MCSA2
Number of Residues	6
Details	M-CSA 642

Chain	Residue	Details
B	LYS549	electrostatic stabiliser
B	THR550	electrostatic stabiliser
B	ASP603	electrostatic stabiliser
B	ASP604	proton acceptor
B	LYS631	electrostatic stabiliser
B	LYS708	electrostatic stabiliser

---

site_id	MCSA3
Number of Residues	6
Details	M-CSA 642

Chain	Residue	Details
C	LYS549	electrostatic stabiliser
C	THR550	electrostatic stabiliser
C	ASP603	electrostatic stabiliser
C	ASP604	proton acceptor
C	LYS631	electrostatic stabiliser
C	LYS708	electrostatic stabiliser

---

site_id	MCSA4
Number of Residues	6
Details	M-CSA 642

Chain	Residue	Details
D	LYS549	electrostatic stabiliser
D	THR550	electrostatic stabiliser
D	ASP603	electrostatic stabiliser
D	ASP604	proton acceptor
D	LYS631	electrostatic stabiliser
D	LYS708	electrostatic stabiliser

---

site_id	MCSA5
Number of Residues	6
Details	M-CSA 642

Chain	Residue	Details
E	LYS549	electrostatic stabiliser
E	THR550	electrostatic stabiliser
E	ASP603	electrostatic stabiliser
E	ASP604	proton acceptor
E	LYS631	electrostatic stabiliser
E	LYS708	electrostatic stabiliser

---

site_id	MCSA6
Number of Residues	6
Details	M-CSA 642

Chain	Residue	Details
F	LYS549	electrostatic stabiliser
F	THR550	electrostatic stabiliser
F	ASP603	electrostatic stabiliser
F	ASP604	proton acceptor
F	LYS631	electrostatic stabiliser
F	LYS708	electrostatic stabiliser

---