3J99
Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State IIIb)
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK I |
Method | Blot for 3.5 seconds before plunging. |
EM Data Collection
Microscope | FEI POLARA 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.3 |
Detector | GATAN K2 (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 8.2 |
Software | PHENIX,UCSF Chimera,RELION |
Symmetry | C1 |