3J1O
Cryo-EM map of a yeast minimal preinitiation complex interacting with the Mediator Head module
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (H) | Mediator of RNA polymerase II transcription subunit 11 | polymer | 131 | 15100.1 | 1 | UniProt (A0A0D3YMY9) Pfam (PF10280) UniProt (by SIFTS) (Q99278) | Saccharomyces cerevisiae (yeast) | Mediator complex subunit 11 |
| 2 | B (I) | Mediator of RNA polymerase II transcription subunit 17 | polymer | 484 | 54893.5 | 1 | UniProt (P32569) Pfam (PF10156) | Saccharomyces cerevisiae (yeast) | Mediator complex subunit 17, Suppressor of RNA polymerase B 4 |
| 3 | C (J) | Mediator of RNA polymerase II transcription subunit 8 | polymer | 223 | 25297.2 | 1 | UniProt (P38304) Pfam (PF10232) | Saccharomyces cerevisiae (yeast) | Mediator complex subunit 8 |
| 4 | D (K) | Mediator of RNA polymerase II transcription subunit 22 | polymer | 121 | 13875.7 | 1 | UniProt (P32570) Pfam (PF06179) | Saccharomyces cerevisiae (yeast) | Mediator complex subunit 22, Suppressor of RNA polymerase B 6 |
| 5 | E (L) | Mediator of RNA polymerase II transcription subunit 18 | polymer | 275 | 30659.8 | 1 | UniProt (P32585) Pfam (PF09637) | Saccharomyces cerevisiae (yeast) | Mediator complex subunit 18, Suppressor of RNA polymerase B 5 |
| 6 | F (M) | Mediator of RNA polymerase II transcription subunit 20 | polymer | 210 | 22918.1 | 1 | UniProt (P34162) Pfam (PF08612) | Saccharomyces cerevisiae (yeast) | Hyper-recombination suppressor protein 2, Mediator complex subunit 20, Suppressor of RNA polymerase B 2 |
| 7 | G (N) | Mediator of RNA polymerase II transcription subunit 6 | polymer | 25 | 2949.4 | 1 | UniProt (P38782) | Saccharomyces cerevisiae (yeast) | Mediator complex subunit 6 |
Sequence modifications
H: 1 - 131 (UniProt: A0A0D3YMY9)
I: 197 - 616 (UniProt: P32569)
I: 669 - 687 (UniProt: P32569)
L: 1 - 140 (UniProt: P32585)
L: 141 - 307 (UniProt: P32585)
| PDB | External Database | Details |
|---|---|---|
| Gly 16 | Thr 16 | engineered mutation |
| Ser 17 | Met 17 | engineered mutation |
I: 669 - 687 (UniProt: P32569)
L: 1 - 140 (UniProt: P32585)
L: 141 - 307 (UniProt: P32585)
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 165693.8 | |
| All* | Total formula weight | 165693.8 |
