3J1O
Cryo-EM map of a yeast minimal preinitiation complex interacting with the Mediator Head module
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
| Method | Blot for ~2 sec before plunging |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 120 |
| Cs (mm) | 2 |
| Detector | TVIPS TEMCAM-F415 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 16 |
| Software | OTHER,SPARX,SPIDER |
| Symmetry | C1 |
